@inbook{9ba4f1ad074a4a1ab2edaae481b37fdc,
title = "Inducible, selective labeling of proteins via enzymatic oxidation of tyrosine",
abstract = "Proteins can be labeled site-specifically and in inducible fashion by exposing a small peptide tag (G4Y) on any of its termini and activating the newly exposed tyrosine residue with the enzyme mushroom tyrosinase. The enzyme generates a quinone by oxidizing the tyrosine, which in turn can perform strain-promoted oxidation-controlled ortho-quinone cycloaddition (SPOCQ) with strained alkynes and alkenes, generating a stable conjugation product. Here, we describe a protocol to perform SPOCQ reaction on proteins, along with notes to optimize yield and reaction rates. Conjugation efficiencies of over 95% to antibodies have been reported using this protocol.",
keywords = "Antibody–drug conjugates (ADC), Bioconjugate chemistry, Bioorthogonal click, Protein functionalization, SPOCQ, Tyrosine modification",
author = "Bruins, {Jorick J.} and {van de Wouw}, Criss and Keijzer, {Jordi F.} and Bauke Albada and {van Delft}, {Floris L.}",
year = "2019",
month = jun,
day = "4",
doi = "10.1007/978-1-4939-9546-2_18",
language = "English",
isbn = "9781493995455",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "357--368",
booktitle = "Methods in Molecular Biology",
address = "Germany",
}