The invention relates to the production of recombinant proteins that are expressed in heterologous host cells and to methods to increase production levels of these proteins by the use of a chaperon-like protein. Producing or isolating a protein or polypeptide in large amounts and in a relatively pure form from its original (homologous) host or host cell where it is expressed is cumbersome and often economically unattractive. However, the development of recombinant DNA methodology has opened the possibility to produce proteins by expression, e.g. in a heterologous host or host cell. A wide and still expanding array of foreign polypeptides or proteins can now be produced or expressed in heterologous expression systems. The invention provides a method to express a polypeptide encoded by a nucleic acid sequence present in a host cell which host cell additionally comprises a chaperone-like protein capable of specific binding with said polypeptide.
|Publication status||Published - 17 Dec 1998|
Harmsen, M., & de Geus, B. (1998). Increased production of proteins by using chaparone-like proteins. (Patent No. WO9856928). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=9856928A1&KC=A1&FT=D&ND=3&date=19981217&DB=&locale=en_EP#