Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry

C.M. Dierikx, A. van Essen-Zandbergen, K.T. Veldman, H.E. Smith, D.J. Mevius

Research output: Contribution to journalArticleAcademicpeer-review

122 Citations (Scopus)

Abstract

To gain more information on the genetic basis of the rapid increase in the number of isolates exhibiting non-wild type Minimum Inhibitory Concentrations (MICs) for cefotaxime observed since 2003, beta-lactamase genes of 22 Salmonella enterica and 22 Escherichia coli isolates from broilers in 2006 showing this phenotype were characterized by miniaturized micro-array, PCR and DNA-sequencing. Presence and size of plasmids were determined by S1-digest pulsed-field gel electrophoresis and further characterized by PCR-based replicon typing. Transfer of resistance plasmids was tested by conjugation and transformation experiments. To link resistance genes and plasmid type, Southern blot hybridization experiments were conducted. In 42 isolates, five (blaCTX-M-1, blaCTX-M-2, blaTEM-20, blaTEM-52, blaSHV-2) different extended spectrum beta-lactamase (ESBL)-genes and two (blaACC-1, blaCMY-2) AmpC-genes were present. Three of the detected ESBL-genes (blaCTX-M-1, blaTEM-52 and blaCTX-M-2) were located on similar types of plasmids (IncI1 and IncHI2/P) in both E. coli and Salmonella. Two other detected ESBL- and AmpC-genes blaSHV-2 and blaCMY-2 respectively (on IncK plasmids), were only found in E. coli, whereas the AmpC-gene blaACC-1 (on non-typable plasmids), and the ESBL-gene blaTEM-20 (on IncI1 plasmids), were only detected in Salmonella. In two isolates, no ESBL- or AmpC-gene could be detected through these methods. The increase in the number of E. coli and S. enterica isolates from the gastro-intestinal tract of broilers exhibiting non-wild type MICs for cefotaxime is mainly due to an increase in IncI1 plasmids containing blaCTX-M-1. The reason for the successful spread of this plasmid type in these species is not yet understood.
Original languageEnglish
Pages (from-to)273-278
JournalVeterinary Microbiology
Volume145
Issue number3-4
DOIs
Publication statusPublished - 2010

Fingerprint

beta-lactamase
Salmonella enterica
beta-Lactamases
Poultry
plasmids
poultry
Plasmids
Escherichia coli
Genes
genes
cefotaxime
Cefotaxime
Microbial Sensitivity Tests
minimum inhibitory concentration
Salmonella
broiler chickens
replicon
Polymerase Chain Reaction
Replicon
Pulsed Field Gel Electrophoresis

Keywords

  • gram-negative bacteria
  • resistance plasmid
  • ctx-m
  • enterobacteriaceae
  • animals
  • cephalosporins
  • identification
  • typhimurium
  • strains
  • genes

Cite this

@article{58189295cf454c6da8a60475426c9e21,
title = "Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry",
abstract = "To gain more information on the genetic basis of the rapid increase in the number of isolates exhibiting non-wild type Minimum Inhibitory Concentrations (MICs) for cefotaxime observed since 2003, beta-lactamase genes of 22 Salmonella enterica and 22 Escherichia coli isolates from broilers in 2006 showing this phenotype were characterized by miniaturized micro-array, PCR and DNA-sequencing. Presence and size of plasmids were determined by S1-digest pulsed-field gel electrophoresis and further characterized by PCR-based replicon typing. Transfer of resistance plasmids was tested by conjugation and transformation experiments. To link resistance genes and plasmid type, Southern blot hybridization experiments were conducted. In 42 isolates, five (blaCTX-M-1, blaCTX-M-2, blaTEM-20, blaTEM-52, blaSHV-2) different extended spectrum beta-lactamase (ESBL)-genes and two (blaACC-1, blaCMY-2) AmpC-genes were present. Three of the detected ESBL-genes (blaCTX-M-1, blaTEM-52 and blaCTX-M-2) were located on similar types of plasmids (IncI1 and IncHI2/P) in both E. coli and Salmonella. Two other detected ESBL- and AmpC-genes blaSHV-2 and blaCMY-2 respectively (on IncK plasmids), were only found in E. coli, whereas the AmpC-gene blaACC-1 (on non-typable plasmids), and the ESBL-gene blaTEM-20 (on IncI1 plasmids), were only detected in Salmonella. In two isolates, no ESBL- or AmpC-gene could be detected through these methods. The increase in the number of E. coli and S. enterica isolates from the gastro-intestinal tract of broilers exhibiting non-wild type MICs for cefotaxime is mainly due to an increase in IncI1 plasmids containing blaCTX-M-1. The reason for the successful spread of this plasmid type in these species is not yet understood.",
keywords = "gram-negative bacteria, resistance plasmid, ctx-m, enterobacteriaceae, animals, cephalosporins, identification, typhimurium, strains, genes",
author = "C.M. Dierikx and {van Essen-Zandbergen}, A. and K.T. Veldman and H.E. Smith and D.J. Mevius",
year = "2010",
doi = "10.1016/j.vetmic.2010.03.019",
language = "English",
volume = "145",
pages = "273--278",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
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Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry. / Dierikx, C.M.; van Essen-Zandbergen, A.; Veldman, K.T.; Smith, H.E.; Mevius, D.J.

In: Veterinary Microbiology, Vol. 145, No. 3-4, 2010, p. 273-278.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry

AU - Dierikx, C.M.

AU - van Essen-Zandbergen, A.

AU - Veldman, K.T.

AU - Smith, H.E.

AU - Mevius, D.J.

PY - 2010

Y1 - 2010

N2 - To gain more information on the genetic basis of the rapid increase in the number of isolates exhibiting non-wild type Minimum Inhibitory Concentrations (MICs) for cefotaxime observed since 2003, beta-lactamase genes of 22 Salmonella enterica and 22 Escherichia coli isolates from broilers in 2006 showing this phenotype were characterized by miniaturized micro-array, PCR and DNA-sequencing. Presence and size of plasmids were determined by S1-digest pulsed-field gel electrophoresis and further characterized by PCR-based replicon typing. Transfer of resistance plasmids was tested by conjugation and transformation experiments. To link resistance genes and plasmid type, Southern blot hybridization experiments were conducted. In 42 isolates, five (blaCTX-M-1, blaCTX-M-2, blaTEM-20, blaTEM-52, blaSHV-2) different extended spectrum beta-lactamase (ESBL)-genes and two (blaACC-1, blaCMY-2) AmpC-genes were present. Three of the detected ESBL-genes (blaCTX-M-1, blaTEM-52 and blaCTX-M-2) were located on similar types of plasmids (IncI1 and IncHI2/P) in both E. coli and Salmonella. Two other detected ESBL- and AmpC-genes blaSHV-2 and blaCMY-2 respectively (on IncK plasmids), were only found in E. coli, whereas the AmpC-gene blaACC-1 (on non-typable plasmids), and the ESBL-gene blaTEM-20 (on IncI1 plasmids), were only detected in Salmonella. In two isolates, no ESBL- or AmpC-gene could be detected through these methods. The increase in the number of E. coli and S. enterica isolates from the gastro-intestinal tract of broilers exhibiting non-wild type MICs for cefotaxime is mainly due to an increase in IncI1 plasmids containing blaCTX-M-1. The reason for the successful spread of this plasmid type in these species is not yet understood.

AB - To gain more information on the genetic basis of the rapid increase in the number of isolates exhibiting non-wild type Minimum Inhibitory Concentrations (MICs) for cefotaxime observed since 2003, beta-lactamase genes of 22 Salmonella enterica and 22 Escherichia coli isolates from broilers in 2006 showing this phenotype were characterized by miniaturized micro-array, PCR and DNA-sequencing. Presence and size of plasmids were determined by S1-digest pulsed-field gel electrophoresis and further characterized by PCR-based replicon typing. Transfer of resistance plasmids was tested by conjugation and transformation experiments. To link resistance genes and plasmid type, Southern blot hybridization experiments were conducted. In 42 isolates, five (blaCTX-M-1, blaCTX-M-2, blaTEM-20, blaTEM-52, blaSHV-2) different extended spectrum beta-lactamase (ESBL)-genes and two (blaACC-1, blaCMY-2) AmpC-genes were present. Three of the detected ESBL-genes (blaCTX-M-1, blaTEM-52 and blaCTX-M-2) were located on similar types of plasmids (IncI1 and IncHI2/P) in both E. coli and Salmonella. Two other detected ESBL- and AmpC-genes blaSHV-2 and blaCMY-2 respectively (on IncK plasmids), were only found in E. coli, whereas the AmpC-gene blaACC-1 (on non-typable plasmids), and the ESBL-gene blaTEM-20 (on IncI1 plasmids), were only detected in Salmonella. In two isolates, no ESBL- or AmpC-gene could be detected through these methods. The increase in the number of E. coli and S. enterica isolates from the gastro-intestinal tract of broilers exhibiting non-wild type MICs for cefotaxime is mainly due to an increase in IncI1 plasmids containing blaCTX-M-1. The reason for the successful spread of this plasmid type in these species is not yet understood.

KW - gram-negative bacteria

KW - resistance plasmid

KW - ctx-m

KW - enterobacteriaceae

KW - animals

KW - cephalosporins

KW - identification

KW - typhimurium

KW - strains

KW - genes

U2 - 10.1016/j.vetmic.2010.03.019

DO - 10.1016/j.vetmic.2010.03.019

M3 - Article

VL - 145

SP - 273

EP - 278

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -