Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures

Gail E. Chapman, Richard Lockey, Katy E. Beck, Chris Vickery, Mark Arnold, Leigh Thorne, Jemma K. Thorne, Sarah R. Walker, Lucien van Keulen, Cristina Casalone, Peter C. Griffiths, Marion M. Simmons, Linda A. Terry, John Spiropoulos*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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The resistance of H-type and L-type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L- and H-type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L-type BSE was reduced by at least 9.40 log10 and of H-type BSE by at least 3.94 log10. In fact, no infectivity was detected for L-type or H-type BSE post-inactivation even at a 10–1 dilution.

Original languageEnglish
Pages (from-to)1872-1878
JournalTransboundary and Emerging Diseases
Issue number5
Early online date15 Feb 2020
Publication statusPublished - Sept 2020


  • bovine spongiform encephalopathy
  • H-type
  • inactivation
  • L-type
  • transmissible spongiform encephalopathie

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