Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures

Gail E. Chapman; Richard Lockey; Katy E. Beck; Chris Vickery; Mark Arnold; Leigh Thorne; Jemma K. Thorne; Sarah R. Walker; Lucien van Keulen; Cristina Casalone; Peter C. Griffiths; Marion M. Simmons; Linda A. Terry; John Spiropoulos

doi:10.1111/tbed.13513

Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures

Gail E. Chapman, Richard Lockey, Katy E. Beck, Chris Vickery, Mark Arnold, Leigh Thorne, Jemma K. Thorne, Sarah R. Walker, Lucien van Keulen, Cristina Casalone, Peter C. Griffiths, Marion M. Simmons, Linda A. Terry, John Spiropoulos^*

^*Corresponding author for this work

Bacteriology, Host Pathogen Interaction & Diagnostics

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

The resistance of H-type and L-type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L- and H-type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L-type BSE was reduced by at least 9.40 log₁₀ and of H-type BSE by at least 3.94 log₁₀. In fact, no infectivity was detected for L-type or H-type BSE post-inactivation even at a 10^–1 dilution.

Original language	English
Pages (from-to)	1872-1878
Journal	Transboundary and Emerging Diseases
Volume	67
Issue number	5
Early online date	15 Feb 2020
DOIs	https://doi.org/10.1111/tbed.13513
Publication status	Published - Sep 2020

Keywords

bovine spongiform encephalopathy
H-type
inactivation
L-type
transmissible spongiform encephalopathie

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10.1111/tbed.13513

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Chapman, G. E., Lockey, R., Beck, K. E., Vickery, C., Arnold, M., Thorne, L., Thorne, J. K., Walker, S. R., van Keulen, L., Casalone, C., Griffiths, P. C., Simmons, M. M., Terry, L. A., & Spiropoulos, J. (2020). Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures. Transboundary and Emerging Diseases, 67(5), 1872-1878. https://doi.org/10.1111/tbed.13513

@article{5f401b9d3f824eb1b1860c3ac6df916e,

title = "Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures",

abstract = "The resistance of H-type and L-type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L- and H-type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L-type BSE was reduced by at least 9.40 log10 and of H-type BSE by at least 3.94 log10. In fact, no infectivity was detected for L-type or H-type BSE post-inactivation even at a 10–1 dilution.",

keywords = "bovine spongiform encephalopathy, H-type, inactivation, L-type, transmissible spongiform encephalopathie",

author = "Chapman, {Gail E.} and Richard Lockey and Beck, {Katy E.} and Chris Vickery and Mark Arnold and Leigh Thorne and Thorne, {Jemma K.} and Walker, {Sarah R.} and {van Keulen}, Lucien and Cristina Casalone and Griffiths, {Peter C.} and Simmons, {Marion M.} and Terry, {Linda A.} and John Spiropoulos",

year = "2020",

month = sep,

doi = "10.1111/tbed.13513",

language = "English",

volume = "67",

pages = "1872--1878",

journal = "Transboundary and Emerging Diseases",

issn = "1865-1674",

publisher = "Wiley",

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Chapman, GE, Lockey, R, Beck, KE, Vickery, C, Arnold, M, Thorne, L, Thorne, JK, Walker, SR, van Keulen, L, Casalone, C, Griffiths, PC, Simmons, MM, Terry, LA & Spiropoulos, J 2020, 'Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures', Transboundary and Emerging Diseases, vol. 67, no. 5, pp. 1872-1878. https://doi.org/10.1111/tbed.13513

TY - JOUR

T1 - Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures

AU - Chapman, Gail E.

AU - Lockey, Richard

AU - Beck, Katy E.

AU - Vickery, Chris

AU - Arnold, Mark

AU - Thorne, Leigh

AU - Thorne, Jemma K.

AU - Walker, Sarah R.

AU - van Keulen, Lucien

AU - Casalone, Cristina

AU - Griffiths, Peter C.

AU - Simmons, Marion M.

AU - Terry, Linda A.

AU - Spiropoulos, John

PY - 2020/9

Y1 - 2020/9

N2 - The resistance of H-type and L-type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L- and H-type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L-type BSE was reduced by at least 9.40 log10 and of H-type BSE by at least 3.94 log10. In fact, no infectivity was detected for L-type or H-type BSE post-inactivation even at a 10–1 dilution.

AB - The resistance of H-type and L-type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L- and H-type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L-type BSE was reduced by at least 9.40 log10 and of H-type BSE by at least 3.94 log10. In fact, no infectivity was detected for L-type or H-type BSE post-inactivation even at a 10–1 dilution.

KW - bovine spongiform encephalopathy

KW - H-type

KW - inactivation

KW - L-type

KW - transmissible spongiform encephalopathie

U2 - 10.1111/tbed.13513

DO - 10.1111/tbed.13513

M3 - Article

AN - SCOPUS:85081238868

VL - 67

SP - 1872

EP - 1878

JO - Transboundary and Emerging Diseases

JF - Transboundary and Emerging Diseases

SN - 1865-1674

IS - 5

ER -

Inactivation of H-type and L-type bovine spongiform encephalopathy following recommended autoclave decontamination procedures

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Keywords

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