Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos

Christa Testerink*, Marco Vennik, Jan W. Kijne, Mei Wang, Sjoukje Heimovaara-Dijkstra

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)55-59
Number of pages5
JournalFEBS Letters
Volume484
Issue number1
DOIs
Publication statusPublished - 27 Oct 2000
Externally publishedYes

Keywords

  • Germination
  • Hordeum distichum
  • Mitogen-activated protein kinase
  • Myelin basic protein kinase
  • Rehydration

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