Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos

Christa Testerink; Marco Vennik; Jan W. Kijne; Mei Wang; Sjoukje Heimovaara-Dijkstra

doi:10.1016/S0014-5793(00)02112-8

Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos

Christa Testerink^*, Marco Vennik, Jan W. Kijne, Mei Wang, Sjoukje Heimovaara-Dijkstra

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos. (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	55-59
Number of pages	5
Journal	FEBS Letters
Volume	484
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)02112-8
Publication status	Published - 27 Oct 2000
Externally published	Yes

Keywords

Germination
Hordeum distichum
Mitogen-activated protein kinase
Myelin basic protein kinase
Rehydration

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title = "Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos",

abstract = "We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos. (C) 2000 Federation of European Biochemical Societies.",

keywords = "Germination, Hordeum distichum, Mitogen-activated protein kinase, Myelin basic protein kinase, Rehydration",

author = "Christa Testerink and Marco Vennik and Kijne, {Jan W.} and Mei Wang and Sjoukje Heimovaara-Dijkstra",

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TY - JOUR

T1 - Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos

AU - Testerink, Christa

AU - Vennik, Marco

AU - Kijne, Jan W.

AU - Wang, Mei

AU - Heimovaara-Dijkstra, Sjoukje

PY - 2000/10/27

Y1 - 2000/10/27

N2 - We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos. (C) 2000 Federation of European Biochemical Societies.

AB - We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos. (C) 2000 Federation of European Biochemical Societies.

KW - Germination

KW - Hordeum distichum

KW - Mitogen-activated protein kinase

KW - Myelin basic protein kinase

KW - Rehydration

U2 - 10.1016/S0014-5793(00)02112-8

DO - 10.1016/S0014-5793(00)02112-8

M3 - Article

C2 - 11056221

AN - SCOPUS:0034721893

VL - 484

SP - 55

EP - 59

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -

Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos

Abstract

Keywords

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