In vivo screening of five phytochemicals/extracts and a fungal immunomodulatory protein against colibacillosis in broilers

H.W. Peek, S.B.A. Halkes, M.M.M. Tomassen, J.J. Mes, W.J.M. Landman

    Research output: Contribution to journalArticleAcademicpeer-review

    4 Citations (Scopus)

    Abstract

    Five phytochemicals/extracts (an extract from Echinacea purpurea, a ß-glucan-rich extract from Shiitake, betaine [Betain™], curcumin from Curcuma longa [turmeric] powder, carvacrol and also a recombinant fungal immunomodulatory protein [FIP] from Ganoderma lucidum) cloned and expressed in Escherichia coli were investigated for their anticolibacillosis potential in three chicken experiments, which were conducted in floor pens. Birds that were inoculated with E. coli intratracheally were treated with the phytochemicals/extracts or the FIP and compared with doxycycline-medicated and non-medicated infected broilers. Non-medicated and non-infected birds were used as negative controls. Mortality, colibacillosis lesions and body weight gains were used as parameters. Considering the sum of dead birds and chickens with generalized colibacillosis per group, there was no significant difference between the positive control groups and birds treated with phytochemicals/extracts or the FIP. In contrast, doxycycline-treated birds showed significantly lower mortality and generalized colibacillosis. Moreover, none of the phytochemicals/extracts and the FIP improved recovery from colibacillosis lesions, while all doxycycline-treated broilers recovered completely. The negative control birds and doxycycline-treated groups consistently showed the highest weight gains. Pulsed-field gel electrophoresis of reisolates showed that they were genetically indistinguishable from the inoculation strain. In conclusion, none of the tested phytochemicals/extracts and the FIP significantly reduced the E. coli-induced mortality and generalized colibacillosis, and nor did they improve recovery from colibacillosis lesions.
    Original languageEnglish
    Pages (from-to)235-247
    JournalAvian Pathology
    Volume42
    Issue number3
    DOIs
    Publication statusPublished - 2013

    Keywords

    • infectious-bronchitis virus
    • eimeria-tenella
    • immune-responses
    • dietary betaine
    • antimicrobial resistance
    • herb polysaccharides
    • commercial broilers
    • ganoderma-lucidium
    • escherichia-coli
    • chicks

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