Abstract
Despite the discovery of many cytokine genes in fish, knowledge on their functional homology is limited. To enlighten the biological function of inflammation-related mediators, we studied their kinetics of gene expression during peritonitis in carp. Zymosan-induced intraperitoneal influx of phagocytes reached a maximum at 24 h. In peritoneal leukocytes (PTL) up-regulation of IL-1ß, TNF-¿, CXCa, and chemokine receptor CXCR1 preceded this peak. Delayed up-regulation of these genes in the head kidney (HK) indicates emigration of antigen-presenting cells from peritoneum into HK and/or systemic spreading of inflammation. In turn, early increase in expression of anti-inflammatory genes in HK (6 h) precede their up-regulation in the focus of inflammation. In PTL peaks of IL-10 and arginase 2 expression were recorded at 96 and 168 h, respectively. These results give evidence that carp macrophages in vivo differentiate into a continuum of different activation states with innate and alternative activation representing the extremes.
Original language | English |
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Pages (from-to) | 509-518 |
Number of pages | 10 |
Journal | Developmental and Comparative Immunology |
Volume | 32 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2008 |
Keywords
- trout oncorhynchus-mykiss
- tumor-necrosis-factor
- rainbow-trout
- common carp
- functional-characterization
- differential expression
- inflammatory responses
- carassius-auratus
- molecular-cloning
- atlantic salmon