In vivo identification of plant protein complexes using IP-MS/MS

Jos R. Wendrich, Sjef Boeren, Barbara Möller, Dolf Weijers, Bert de Rybel

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Citations (Scopus)

Abstract

Individual proteins often function as part of a protein complex. The identification of interacting proteins is therefore vital to understand the biological role and function of the studied protein. Here we describe a method for the in vivo identification of nuclear, cytoplasmic, and membrane-associated protein complexes from plant tissues using a strategy of immunoprecipitation followed by tandem mass spectrometry. By performing quantitative mass spectrometry measurements on biological triplicates, relative abundance of proteins in GFP-tagged complexes compared to background controls can be statistically evaluated to identify high-confidence interactors. We detail the entire workflow of this approach.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsJürgen Kleine-Vehn, Michael Sauer
PublisherHumana Press Inc.
Pages147-158
Volume1497
ISBN (Electronic)9781493964697
ISBN (Print)9781493964673
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1497
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • GFP
  • Immunoprecipitation
  • Protein complex identification
  • Protein–protein interaction
  • Tandem mass spectrometry

Fingerprint Dive into the research topics of 'In vivo identification of plant protein complexes using IP-MS/MS'. Together they form a unique fingerprint.

  • Cite this

    Wendrich, J. R., Boeren, S., Möller, B., Weijers, D., & de Rybel, B. (2017). In vivo identification of plant protein complexes using IP-MS/MS. In J. Kleine-Vehn, & M. Sauer (Eds.), Methods in Molecular Biology (Vol. 1497, pp. 147-158). (Methods in Molecular Biology; Vol. 1497). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6469-7_14