In vivo identification of plant protein complexes using IP-MS/MS

Jos R. Wendrich, Sjef Boeren, Barbara Möller, Dolf Weijers, Bert de Rybel

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

43 Citations (Scopus)


Individual proteins often function as part of a protein complex. The identification of interacting proteins is therefore vital to understand the biological role and function of the studied protein. Here we describe a method for the in vivo identification of nuclear, cytoplasmic, and membrane-associated protein complexes from plant tissues using a strategy of immunoprecipitation followed by tandem mass spectrometry. By performing quantitative mass spectrometry measurements on biological triplicates, relative abundance of proteins in GFP-tagged complexes compared to background controls can be statistically evaluated to identify high-confidence interactors. We detail the entire workflow of this approach.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsJürgen Kleine-Vehn, Michael Sauer
PublisherHumana Press
ISBN (Electronic)9781493964697
ISBN (Print)9781493964673
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Arabidopsis
  • GFP
  • Immunoprecipitation
  • Protein complex identification
  • Protein–protein interaction
  • Tandem mass spectrometry


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