Abstract
Scope: To study the effect of residual myrosinase (MYR) activity in differently processed
broccoli on sulforaphane (SR) and iberin (IB) formation, bioavailability, and excretion in human
volunteers.
Methods and results: Five different broccoli products were obtainedwith similar glucoraphanin
(GR) and glucoiberin (GI) content, yet different MYR activity. Excretion of SR and IB conjugates
in urine were determined in 15 participants after ingestion of the broccoli products. A reduction
of 80% of MYR in the product did not cause differences in the total amount of SR and IB found
in urine compared to the product with 100% MYR. Complete inactivation of MYR gave the
lowest total amount of SR and IB in urine (10 and 19%). A residual MYR of only 2% in the
product gave an intermediate amount (17 and 29%). The excretion half-lives of SR and IB
conjugates were comparable for all the products (2.5 h on average), although the maximum
excretion peak times were clearly shorter when the residual MYR was higher (2.3–6.1 h).
Conclusion: For the first time, the effect of residual MYR activity on isothiocyanate bioavailability
was systematically and quantitatively studied. Processing conditions have a large effect
on the kinetics and bioavailability of isothiocyanates from broccoli.
Original language | English |
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Pages (from-to) | 1447-1456 |
Journal | Molecular Nutrition & Food Research |
Volume | 58 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- brassica vegetables
- cruciferous vegetables
- myrosinase activity
- human health
- red cabbage
- sulforaphane
- excretion
- l.
- degradation
- consumption