In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway

A.R. Companjen, B. Heinhuis, K. Aspers, J.H.W.M. Rombout

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes
Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalFish and Shellfish Immunology
Volume20
Issue number1
DOIs
Publication statusPublished - 2006

Keywords

  • ginbuna crucian carp
  • sparus-aurata l.
  • fas ligand
  • ictalurus-punctatus
  • allogeneic targets
  • fish
  • identification
  • mechanisms
  • leukocytes
  • induction

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