In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway

A.R. Companjen; B. Heinhuis; K. Aspers; J.H.W.M. Rombout

doi:10.1016/j.fsi.2005.03.009

In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway

A.R. Companjen, B. Heinhuis, K. Aspers, J.H.W.M. Rombout

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes

Original language	English
Pages (from-to)	113-117
Number of pages	5
Journal	Fish and Shellfish Immunology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/j.fsi.2005.03.009
Publication status	Published - 2006

Keywords

ginbuna crucian carp
sparus-aurata l.
fas ligand
ictalurus-punctatus
allogeneic targets
fish
identification
mechanisms
leukocytes
induction

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10.1016/j.fsi.2005.03.009

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title = "In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway",

abstract = "A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes",

keywords = "ginbuna crucian carp, sparus-aurata l., fas ligand, ictalurus-punctatus, allogeneic targets, fish, identification, mechanisms, leukocytes, induction",

author = "A.R. Companjen and B. Heinhuis and K. Aspers and J.H.W.M. Rombout",

year = "2006",

doi = "10.1016/j.fsi.2005.03.009",

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T1 - In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway

AU - Companjen, A.R.

AU - Heinhuis, B.

AU - Aspers, K.

AU - Rombout, J.H.W.M.

PY - 2006

Y1 - 2006

N2 - A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes

AB - A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes

KW - ginbuna crucian carp

KW - sparus-aurata l.

KW - fas ligand

KW - ictalurus-punctatus

KW - allogeneic targets

KW - fish

KW - identification

KW - mechanisms

KW - leukocytes

KW - induction

U2 - 10.1016/j.fsi.2005.03.009

DO - 10.1016/j.fsi.2005.03.009

M3 - Article

SN - 1050-4648

VL - 20

SP - 113

EP - 117

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

IS - 1

ER -

In vivo evoked specific cell mediated cytotoxicity in carp (Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway

Abstract

Keywords

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