In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation

J.B. Kim; M.J. de Jeu; C.J.J.M. Raemakers; E. Jacobsen; R.G.F. Visser

In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation

J.B. Kim, M.J. de Jeu, C.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

Abstract

We compared both vegetative and generative parts of Alstroemeria in friable embryogenic callus induction, and subsequent production of somatic embryos and regeneration of plants because there exist only a few protocols with low efficiency for application to transformation. Nodal parts of the plant showed the best performance in callus induction, production of somatic embryos and regeneration as compared to internodes and all seven generative parts tested. Thus, it can be concluded that the nodal explants can be used as a source for transformation in combination with the MS medium containing 1 mg/l 2,4-D, 0.25 mg/l BAP, 3␜ucrose (w/v) and 0.75øw/v) microagar for the production of a high level of compact callus and somatic embryos. In the future, more research will be needed on the acceleration of the plant production process from somatic embryos, to use this system in an optimised way for transformation experiments.

Original language	English
Title of host publication	Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland
Editors	S. Sorvari, S. Karhu, E. Kanervo, S. Pihakaski
Pages	437-441
Publication status	Published - 2001

Publication series

Name	Acta Horticulturae
Number	560
ISSN (Print)	0567-7572

Access to Document

https://edepot.wur.nl/44996

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Kim, J. B., de Jeu, M. J., Raemakers, C. J. J. M., Jacobsen, E., & Visser, R. G. F. (2001). In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation. In S. Sorvari, S. Karhu, E. Kanervo, & S. Pihakaski (Eds.), Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland (pp. 437-441). (Acta Horticulturae; No. 560). https://edepot.wur.nl/44996

Kim, J.B. ; de Jeu, M.J. ; Raemakers, C.J.J.M. ; Jacobsen, E. ; Visser, R.G.F. / In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation. Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland. editor / S. Sorvari ; S. Karhu ; E. Kanervo ; S. Pihakaski. 2001. pp. 437-441 (Acta Horticulturae; 560).

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title = "In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation",

abstract = "We compared both vegetative and generative parts of Alstroemeria in friable embryogenic callus induction, and subsequent production of somatic embryos and regeneration of plants because there exist only a few protocols with low efficiency for application to transformation. Nodal parts of the plant showed the best performance in callus induction, production of somatic embryos and regeneration as compared to internodes and all seven generative parts tested. Thus, it can be concluded that the nodal explants can be used as a source for transformation in combination with the MS medium containing 1 mg/l 2,4-D, 0.25 mg/l BAP, 3␜ucrose (w/v) and 0.75{\o}w/v) microagar for the production of a high level of compact callus and somatic embryos. In the future, more research will be needed on the acceleration of the plant production process from somatic embryos, to use this system in an optimised way for transformation experiments.",

author = "J.B. Kim and {de Jeu}, M.J. and C.J.J.M. Raemakers and E. Jacobsen and R.G.F. Visser",

year = "2001",

language = "English",

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series = "Acta Horticulturae",

number = "560",

pages = "437--441",

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Kim, JB, de Jeu, MJ, Raemakers, CJJM, Jacobsen, E & Visser, RGF 2001, In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation. in S Sorvari, S Karhu, E Kanervo & S Pihakaski (eds), Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland. Acta Horticulturae, no. 560, pp. 437-441. <https://edepot.wur.nl/44996>

In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation. / Kim, J.B.; de Jeu, M.J.; Raemakers, C.J.J.M.; Jacobsen, E.; Visser, R.G.F.

Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland. ed. / S. Sorvari; S. Karhu; E. Kanervo; S. Pihakaski. 2001. p. 437-441 (Acta Horticulturae; No. 560).

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

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T1 - In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation

AU - Kim, J.B.

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N2 - We compared both vegetative and generative parts of Alstroemeria in friable embryogenic callus induction, and subsequent production of somatic embryos and regeneration of plants because there exist only a few protocols with low efficiency for application to transformation. Nodal parts of the plant showed the best performance in callus induction, production of somatic embryos and regeneration as compared to internodes and all seven generative parts tested. Thus, it can be concluded that the nodal explants can be used as a source for transformation in combination with the MS medium containing 1 mg/l 2,4-D, 0.25 mg/l BAP, 3␜ucrose (w/v) and 0.75øw/v) microagar for the production of a high level of compact callus and somatic embryos. In the future, more research will be needed on the acceleration of the plant production process from somatic embryos, to use this system in an optimised way for transformation experiments.

AB - We compared both vegetative and generative parts of Alstroemeria in friable embryogenic callus induction, and subsequent production of somatic embryos and regeneration of plants because there exist only a few protocols with low efficiency for application to transformation. Nodal parts of the plant showed the best performance in callus induction, production of somatic embryos and regeneration as compared to internodes and all seven generative parts tested. Thus, it can be concluded that the nodal explants can be used as a source for transformation in combination with the MS medium containing 1 mg/l 2,4-D, 0.25 mg/l BAP, 3␜ucrose (w/v) and 0.75øw/v) microagar for the production of a high level of compact callus and somatic embryos. In the future, more research will be needed on the acceleration of the plant production process from somatic embryos, to use this system in an optimised way for transformation experiments.

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BT - Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland

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Kim JB, de Jeu MJ, Raemakers CJJM, Jacobsen E , Visser RGF. In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation. In Sorvari S, Karhu S, Kanervo E, Pihakaski S, editors, Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland. 2001. p. 437-441. (Acta Horticulturae; 560).