In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation

J.B. Kim, M.J. de Jeu, C.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser

Research output: Chapter in Book/Report/Conference proceedingConference paper

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Abstract

We compared both vegetative and generative parts of Alstroemeria in friable embryogenic callus induction, and subsequent production of somatic embryos and regeneration of plants because there exist only a few protocols with low efficiency for application to transformation. Nodal parts of the plant showed the best performance in callus induction, production of somatic embryos and regeneration as compared to internodes and all seven generative parts tested. Thus, it can be concluded that the nodal explants can be used as a source for transformation in combination with the MS medium containing 1 mg/l 2,4-D, 0.25 mg/l BAP, 3␜ucrose (w/v) and 0.75øw/v) microagar for the production of a high level of compact callus and somatic embryos. In the future, more research will be needed on the acceleration of the plant production process from somatic embryos, to use this system in an optimised way for transformation experiments.
Original languageEnglish
Title of host publicationProceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland
EditorsS. Sorvari, S. Karhu, E. Kanervo, S. Pihakaski
Pages437-441
Publication statusPublished - 2001

Publication series

NameActa Horticulturae
Number560
ISSN (Print)0567-7572

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Kim, J. B., de Jeu, M. J., Raemakers, C. J. J. M., Jacobsen, E., & Visser, R. G. F. (2001). In vitro studies on callus induction in both vegetative and generative parts in Alstroemeria for further application to transformation. In S. Sorvari, S. Karhu, E. Kanervo, & S. Pihakaski (Eds.), Proceedings of the Fourth International Symposium on In vitro culture and Horticultural Breeding, Tampere, Finland (pp. 437-441). (Acta Horticulturae; No. 560).