In vitro rearing of honey bees at Bees@wur

C. van Dooremalen, E. Stam, J. Zoeteweij, J.J.M. van der Steen, B. Cornelissen

    Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

    Abstract

    The rearing of larvae in a laboratory (in vitro) is highly attractive because of controlled laboratory conditions and the reproducibility. Biologically relevant factors such as weight, development (e.g. hypopharyngeal gland) and the survival of the larvae, or longevity and behaviour (e.g. flight performance) of artificially reared adult workers can be easily monitored under laboratory conditions. Hence, a large advantage of in vitro rearing of larvae is that feedback mechanisms on the colony level can be excluded to study the pure effects of the factors of interest. Many in vitro tests are hampered by high mortality of the test subjects, lack of standardization and repeatability. In our lab, we are able to rear larvae up to day 6, but after that, they die and turn black. Out of the >2000 larvae we started to rear between April and October 2011, we only managed to rear one sad little bee, which died a few days after it was born. We used the protocol of Aupinel (2005), occasionally using the nicotplast egg laying method from Hendriksma (2011). During the season, we observed differences in laying behaviour between colonies. Some queens refused to lay eggs in the nicotplast, even when the nicotplast were waxcoated. Towards the end of the bee season, survivability of the larvae decreased, which could be a natural phenomenon. Within batches, we observed that that units (wells plates) that were filled with crafted larvae first and were subsequently fed first, showed higher larval survival than units crafted and fed last. We assume that larvae crafted last, were longer exposed to the cold and therefore less vital. Additionally, due to the cooling down of feed during feeding, larvae fed last suffer from cold food. Conclusion, craft fast and rewarm food between units
    LanguageEnglish
    Title of host publicationProceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011
    EditorsP. Aupinel, M.P. Chauzat
    PublisherCOST Action FA0803
    Pages19
    Publication statusPublished - 2011
    EventCOLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France -
    Duration: 17 Nov 201119 Nov 2011

    Workshop

    WorkshopCOLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France
    Period17/11/1119/11/11

    Fingerprint

    honey bees
    Apoidea
    rearing
    larvae
    handicrafts
    queen insects
    standardization
    reproducibility
    repeatability
    oviposition
    flight
    cooling
    testing

    Cite this

    van Dooremalen, C., Stam, E., Zoeteweij, J., van der Steen, J. J. M., & Cornelissen, B. (2011). In vitro rearing of honey bees at Bees@wur. In P. Aupinel, & M. P. Chauzat (Eds.), Proceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011 (pp. 19). COST Action FA0803.
    van Dooremalen, C. ; Stam, E. ; Zoeteweij, J. ; van der Steen, J.J.M. ; Cornelissen, B. / In vitro rearing of honey bees at Bees@wur. Proceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011. editor / P. Aupinel ; M.P. Chauzat. COST Action FA0803, 2011. pp. 19
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    title = "In vitro rearing of honey bees at Bees@wur",
    abstract = "The rearing of larvae in a laboratory (in vitro) is highly attractive because of controlled laboratory conditions and the reproducibility. Biologically relevant factors such as weight, development (e.g. hypopharyngeal gland) and the survival of the larvae, or longevity and behaviour (e.g. flight performance) of artificially reared adult workers can be easily monitored under laboratory conditions. Hence, a large advantage of in vitro rearing of larvae is that feedback mechanisms on the colony level can be excluded to study the pure effects of the factors of interest. Many in vitro tests are hampered by high mortality of the test subjects, lack of standardization and repeatability. In our lab, we are able to rear larvae up to day 6, but after that, they die and turn black. Out of the >2000 larvae we started to rear between April and October 2011, we only managed to rear one sad little bee, which died a few days after it was born. We used the protocol of Aupinel (2005), occasionally using the nicotplast egg laying method from Hendriksma (2011). During the season, we observed differences in laying behaviour between colonies. Some queens refused to lay eggs in the nicotplast, even when the nicotplast were waxcoated. Towards the end of the bee season, survivability of the larvae decreased, which could be a natural phenomenon. Within batches, we observed that that units (wells plates) that were filled with crafted larvae first and were subsequently fed first, showed higher larval survival than units crafted and fed last. We assume that larvae crafted last, were longer exposed to the cold and therefore less vital. Additionally, due to the cooling down of feed during feeding, larvae fed last suffer from cold food. Conclusion, craft fast and rewarm food between units",
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    van Dooremalen, C, Stam, E, Zoeteweij, J, van der Steen, JJM & Cornelissen, B 2011, In vitro rearing of honey bees at Bees@wur. in P Aupinel & MP Chauzat (eds), Proceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011. COST Action FA0803, pp. 19, COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 17/11/11.

    In vitro rearing of honey bees at Bees@wur. / van Dooremalen, C.; Stam, E.; Zoeteweij, J.; van der Steen, J.J.M.; Cornelissen, B.

    Proceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011. ed. / P. Aupinel; M.P. Chauzat. COST Action FA0803, 2011. p. 19.

    Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

    TY - CHAP

    T1 - In vitro rearing of honey bees at Bees@wur

    AU - van Dooremalen, C.

    AU - Stam, E.

    AU - Zoeteweij, J.

    AU - van der Steen, J.J.M.

    AU - Cornelissen, B.

    PY - 2011

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    N2 - The rearing of larvae in a laboratory (in vitro) is highly attractive because of controlled laboratory conditions and the reproducibility. Biologically relevant factors such as weight, development (e.g. hypopharyngeal gland) and the survival of the larvae, or longevity and behaviour (e.g. flight performance) of artificially reared adult workers can be easily monitored under laboratory conditions. Hence, a large advantage of in vitro rearing of larvae is that feedback mechanisms on the colony level can be excluded to study the pure effects of the factors of interest. Many in vitro tests are hampered by high mortality of the test subjects, lack of standardization and repeatability. In our lab, we are able to rear larvae up to day 6, but after that, they die and turn black. Out of the >2000 larvae we started to rear between April and October 2011, we only managed to rear one sad little bee, which died a few days after it was born. We used the protocol of Aupinel (2005), occasionally using the nicotplast egg laying method from Hendriksma (2011). During the season, we observed differences in laying behaviour between colonies. Some queens refused to lay eggs in the nicotplast, even when the nicotplast were waxcoated. Towards the end of the bee season, survivability of the larvae decreased, which could be a natural phenomenon. Within batches, we observed that that units (wells plates) that were filled with crafted larvae first and were subsequently fed first, showed higher larval survival than units crafted and fed last. We assume that larvae crafted last, were longer exposed to the cold and therefore less vital. Additionally, due to the cooling down of feed during feeding, larvae fed last suffer from cold food. Conclusion, craft fast and rewarm food between units

    AB - The rearing of larvae in a laboratory (in vitro) is highly attractive because of controlled laboratory conditions and the reproducibility. Biologically relevant factors such as weight, development (e.g. hypopharyngeal gland) and the survival of the larvae, or longevity and behaviour (e.g. flight performance) of artificially reared adult workers can be easily monitored under laboratory conditions. Hence, a large advantage of in vitro rearing of larvae is that feedback mechanisms on the colony level can be excluded to study the pure effects of the factors of interest. Many in vitro tests are hampered by high mortality of the test subjects, lack of standardization and repeatability. In our lab, we are able to rear larvae up to day 6, but after that, they die and turn black. Out of the >2000 larvae we started to rear between April and October 2011, we only managed to rear one sad little bee, which died a few days after it was born. We used the protocol of Aupinel (2005), occasionally using the nicotplast egg laying method from Hendriksma (2011). During the season, we observed differences in laying behaviour between colonies. Some queens refused to lay eggs in the nicotplast, even when the nicotplast were waxcoated. Towards the end of the bee season, survivability of the larvae decreased, which could be a natural phenomenon. Within batches, we observed that that units (wells plates) that were filled with crafted larvae first and were subsequently fed first, showed higher larval survival than units crafted and fed last. We assume that larvae crafted last, were longer exposed to the cold and therefore less vital. Additionally, due to the cooling down of feed during feeding, larvae fed last suffer from cold food. Conclusion, craft fast and rewarm food between units

    M3 - Abstract

    SP - 19

    BT - Proceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011

    A2 - Aupinel, P.

    A2 - Chauzat, M.P.

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    van Dooremalen C, Stam E, Zoeteweij J, van der Steen JJM, Cornelissen B. In vitro rearing of honey bees at Bees@wur. In Aupinel P, Chauzat MP, editors, Proceedings COLOSS Working Group 3 Workshop “In vitro larval rearing workshop”, La Rochelle, France, 18-19 November 2011. COST Action FA0803. 2011. p. 19