In vitro interactions between Blautia hydrogenotrophica, Desulfovibrio piger and Methanobrevibacter smithii under hydrogenotrophic conditions

Taojun Wang*, Nils Leibrock, Caroline M. Plugge, Hauke Smidt, Erwin G. Zoetendal

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Methanogens, reductive acetogens and sulfate-reducing bacteria play an important role in disposing of hydrogen in gut ecosystems. However, how they interact with each other remains largely unknown. This in vitro study cocultured Blautia hydrogenotrophica (reductive acetogen), Desulfovibrio piger (sulfate reducer) and Methanobrevibacter smithii (methanogen). Results revealed that these three species coexisted and did not compete for hydrogen in the early phase of incubations. Sulfate reduction was not affected by B. hydrogenotrophica and M. smithii. D. piger inhibited the growth of B. hydrogenotrophica and M. smithii after 10 h incubations, and the inhibition on M. smithii was associated with increased sulfide concentration. Remarkably, M. smithii growth lag phase was shortened by coculturing with B. hydrogenotrophica and D. piger. Formate was rapidly used by M. smithii under high acetate concentration. Overall, these findings indicated that the interactions of the hydrogenotrophic microbes are condition-dependent, suggesting their interactions may vary in gut ecosystems.

Original languageEnglish
Article number2261784
JournalGut Microbes
Volume15
Issue number2
DOIs
Publication statusPublished - 27 Sept 2023

Keywords

  • acetate
  • Coculture
  • formate
  • hydrogen
  • methanogen
  • sulfide

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