In vitro gas and methane production of some common feedstuffs used for dairy rations in Vietnam and Thailand

N.T.D. Huyen*, Thomas Schonewille, Wilbert Frans Pellikaan, N.X. Trach, Wouter Hendriks

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)


Determine fermentation characteristics of commonly used feedstuffs especially tropical roughages, for dairy cattle in Southeast Asia. This information is considered relevant in the context of the observed low milk fat content and milk production in Southeast Asia countries.
A total of 29 feedstuffs commonly used for dairy cattle in Vietnam and Thailand were chemically analysed and subjected to an in vitro gas production (GP) test. For 72 h, GP was continuously recorded with fully automated equipment and methane (CH4) was measured at 0, 3, 6, 9, 12, 24, 30, 36, 48, 60, and 72 h of incubation. A triphasic, nonlinear, regression procedure was applied to analyse GP profiles while a monophasic model was used to obtain kinetics related to CH4 production.
King grass and VA06 showed a high asymptotic GP related to the soluble- and non-soluble fraction (i.e. A1 and A2, respectively) and had the highest acetate to propionate ratio in the incubation fluid. The proportion of CH4 produced (% of GP at 72 h) was found to be not different (P > 0.05) between the various grasses. Among the selected preserved roughages (n = 6) and whole crops (n = 4), sorghum was found to produce the greatest amount of gas in combination with a relatively low CH4 production.
Grasses belonging to the genus Pennisetum, and whole crop sorghum can be considered as suitable ingredients to formulate dairy rations to enhance milk fat content in Vietnam/Thailand.
Original languageEnglish
Pages (from-to)481-491
Number of pages11
JournalAnimal Bioscience
Issue number3
Early online date23 Aug 2023
Publication statusPublished - Mar 2024


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