In vitro fermentation kinetics of some non-digestible carbohydrates by the caecal microbial community of broilers

Y. Lan, B.A. Williams, S. Tamminga, H. Boer, A. Akkermans, G. Erdi, M.W.A. Verstegen

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Fermentable carbohydrates can lead to changes in the gut microflora, which may have positive consequences for health. However, often, ingredients are added to diets without prior investigating about their potential fermentability within the target animal. The experiment reported, was conducted to investigate the fermentation kinetics of some non-digestible carbohydrates (NDC) by the caecal microbial community of broiler chickens by an assessment of the cumulative gas production during fermentation of each substrate. It also aimed to study changes in the microbial community, following fermentation of these non-digestible carbohydrates, by use of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Four extracted non-digestible carbohydrates (ENC): soybean meal oligo- and water-soluble polysaccharides (SMO and SMP), alfalfa meal oligo- and water-soluble polysaccharides (AMO and AMP) were studied. Two pure sugars, raffinose (RAF) and stachyose (STA) were also included. To assess the fermentability of the substrates, cumulative gas pressure was monitored continuously, for 72 h, and at the end of fermentation, pH, volatile fatty acid (VFA), and ammonia (NH3) concentrations were measured in the fermentation solution. The PCR-DGGE technique was applied to compare microbial DNA fingerprints between substrates at the end of the fermentation. The inoculum for the in vitro gas production was obtained from a mixture of caecal contents of forty 81-day-old broiler birds. Soy oligosaccharides led to significantly more butyric acid production (P60¿80%) between some of the substrates. It was concluded that SMO, SMP, AMO and AMP, as well as RAF and STA were significantly different, both in terms of their fermentation kinetics and end-products using caecal contents from adult broilers. The extent to which these non-digestible, but fermentable, carbohydrates could change the microbial community of the broiler caecum, either in terms of the species detected, or its activity, needs to be investigated further, and then related to its effect on gut health in the animal itself.
Original languageEnglish
Pages (from-to)687-702
Number of pages16
JournalAnimal Feed Science and Technology
Volume123-124
Issue number2
DOIs
Publication statusPublished - 2005

    Fingerprint

Keywords

  • true metabolizable energy
  • colon-cancer
  • dietary modulation
  • gas-production
  • soybean-meal
  • rumen fluid
  • fatty-acids
  • oligosaccharides
  • nitrogen
  • bacteria

Cite this