In vitro degradation behaviour of biodegradable soy plastics : effects of crosslinking with glyoxal and thermal treatment

C.M. Vaz, L.A. de Graaf, R.L. Reis, A.M. Cunha

    Research output: Contribution to journalArticleAcademicpeer-review

    47 Citations (Scopus)

    Abstract

    In-vitro degradation of soy-derived protein materials, non-crosslinked (SItp), crosslinked with glyoxal (X-SItp) or submitted to heat treatment (24TT-SItp), was studied with either an isotonic saline solution without enzymatic activity or containing bacterial collagenase. The changes in weight of the samples during the in-vitro degradation were studied and compared with the variations of the mechanical properties. The weight loss of SItp, X-SItp and 24TT-SItp were more pronounced when using collagenase. After 24 h of immersion, SItp lost 10.6 f its initial weight whereas 0.6X-SItp and 24TT-SItp lost 1.7 and 5.7°respectively. In every case, the weight loss was found to be directly proportional to the respective crosslinking degree: 2.4 or SItp, 44 or 0.6X-SItp and 27.8 or 24TT-SItp. Consequently, the susceptibility of the soy materials towards enzymatic degradation could be controlled by varying the degree of crosslinking of the samples. The mechanical properties proved to be more sensitive to the loss of plasticiser (glycerol) during immersion than to the degradation of the polymeric matrices. After 24 h of immersion all the materials presented an increase in stiffness and brittleness due to the complete leaching of glycerol from the matrices. SItp, X-SItp and 24TT-SItp proved to be suitable materials for either load-bearing applications or temporary applications such as tissue engineering scaffolds or drug delivery systems.
    Original languageEnglish
    Pages (from-to)65-74
    JournalPolymer Degradation and Stability
    Volume81
    Issue number1
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Glyoxal
    crosslinking
    Crosslinking
    plastics
    Heat treatment
    submerging
    degradation
    Plastics
    Degradation
    Collagenases
    glycerols
    Glycerol
    Bearings (structural)
    mechanical properties
    Tissue Scaffolds
    Plasticizers
    Mechanical properties
    brittleness
    plasticizers
    tissue engineering

    Keywords

    • water-soluble carbodiimide
    • dermal sheep collagens
    • mechanical-properties
    • cross-linking
    • protein
    • glutaraldehyde
    • cytotoxicity
    • composites
    • carbonate
    • release

    Cite this

    @article{67a92c5131ba4790961313e019f80499,
    title = "In vitro degradation behaviour of biodegradable soy plastics : effects of crosslinking with glyoxal and thermal treatment",
    abstract = "In-vitro degradation of soy-derived protein materials, non-crosslinked (SItp), crosslinked with glyoxal (X-SItp) or submitted to heat treatment (24TT-SItp), was studied with either an isotonic saline solution without enzymatic activity or containing bacterial collagenase. The changes in weight of the samples during the in-vitro degradation were studied and compared with the variations of the mechanical properties. The weight loss of SItp, X-SItp and 24TT-SItp were more pronounced when using collagenase. After 24 h of immersion, SItp lost 10.6 f its initial weight whereas 0.6X-SItp and 24TT-SItp lost 1.7 and 5.7°respectively. In every case, the weight loss was found to be directly proportional to the respective crosslinking degree: 2.4 or SItp, 44 or 0.6X-SItp and 27.8 or 24TT-SItp. Consequently, the susceptibility of the soy materials towards enzymatic degradation could be controlled by varying the degree of crosslinking of the samples. The mechanical properties proved to be more sensitive to the loss of plasticiser (glycerol) during immersion than to the degradation of the polymeric matrices. After 24 h of immersion all the materials presented an increase in stiffness and brittleness due to the complete leaching of glycerol from the matrices. SItp, X-SItp and 24TT-SItp proved to be suitable materials for either load-bearing applications or temporary applications such as tissue engineering scaffolds or drug delivery systems.",
    keywords = "water-soluble carbodiimide, dermal sheep collagens, mechanical-properties, cross-linking, protein, glutaraldehyde, cytotoxicity, composites, carbonate, release",
    author = "C.M. Vaz and {de Graaf}, L.A. and R.L. Reis and A.M. Cunha",
    year = "2003",
    doi = "10.1016/S0141-3910(03)00063-6",
    language = "English",
    volume = "81",
    pages = "65--74",
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    }

    In vitro degradation behaviour of biodegradable soy plastics : effects of crosslinking with glyoxal and thermal treatment. / Vaz, C.M.; de Graaf, L.A.; Reis, R.L.; Cunha, A.M.

    In: Polymer Degradation and Stability, Vol. 81, No. 1, 2003, p. 65-74.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - In vitro degradation behaviour of biodegradable soy plastics : effects of crosslinking with glyoxal and thermal treatment

    AU - Vaz, C.M.

    AU - de Graaf, L.A.

    AU - Reis, R.L.

    AU - Cunha, A.M.

    PY - 2003

    Y1 - 2003

    N2 - In-vitro degradation of soy-derived protein materials, non-crosslinked (SItp), crosslinked with glyoxal (X-SItp) or submitted to heat treatment (24TT-SItp), was studied with either an isotonic saline solution without enzymatic activity or containing bacterial collagenase. The changes in weight of the samples during the in-vitro degradation were studied and compared with the variations of the mechanical properties. The weight loss of SItp, X-SItp and 24TT-SItp were more pronounced when using collagenase. After 24 h of immersion, SItp lost 10.6 f its initial weight whereas 0.6X-SItp and 24TT-SItp lost 1.7 and 5.7°respectively. In every case, the weight loss was found to be directly proportional to the respective crosslinking degree: 2.4 or SItp, 44 or 0.6X-SItp and 27.8 or 24TT-SItp. Consequently, the susceptibility of the soy materials towards enzymatic degradation could be controlled by varying the degree of crosslinking of the samples. The mechanical properties proved to be more sensitive to the loss of plasticiser (glycerol) during immersion than to the degradation of the polymeric matrices. After 24 h of immersion all the materials presented an increase in stiffness and brittleness due to the complete leaching of glycerol from the matrices. SItp, X-SItp and 24TT-SItp proved to be suitable materials for either load-bearing applications or temporary applications such as tissue engineering scaffolds or drug delivery systems.

    AB - In-vitro degradation of soy-derived protein materials, non-crosslinked (SItp), crosslinked with glyoxal (X-SItp) or submitted to heat treatment (24TT-SItp), was studied with either an isotonic saline solution without enzymatic activity or containing bacterial collagenase. The changes in weight of the samples during the in-vitro degradation were studied and compared with the variations of the mechanical properties. The weight loss of SItp, X-SItp and 24TT-SItp were more pronounced when using collagenase. After 24 h of immersion, SItp lost 10.6 f its initial weight whereas 0.6X-SItp and 24TT-SItp lost 1.7 and 5.7°respectively. In every case, the weight loss was found to be directly proportional to the respective crosslinking degree: 2.4 or SItp, 44 or 0.6X-SItp and 27.8 or 24TT-SItp. Consequently, the susceptibility of the soy materials towards enzymatic degradation could be controlled by varying the degree of crosslinking of the samples. The mechanical properties proved to be more sensitive to the loss of plasticiser (glycerol) during immersion than to the degradation of the polymeric matrices. After 24 h of immersion all the materials presented an increase in stiffness and brittleness due to the complete leaching of glycerol from the matrices. SItp, X-SItp and 24TT-SItp proved to be suitable materials for either load-bearing applications or temporary applications such as tissue engineering scaffolds or drug delivery systems.

    KW - water-soluble carbodiimide

    KW - dermal sheep collagens

    KW - mechanical-properties

    KW - cross-linking

    KW - protein

    KW - glutaraldehyde

    KW - cytotoxicity

    KW - composites

    KW - carbonate

    KW - release

    U2 - 10.1016/S0141-3910(03)00063-6

    DO - 10.1016/S0141-3910(03)00063-6

    M3 - Article

    VL - 81

    SP - 65

    EP - 74

    JO - Polymer Degradation and Stability

    JF - Polymer Degradation and Stability

    SN - 0141-3910

    IS - 1

    ER -