In Vitro conservation of enset under slow growth conditions

    Research output: Contribution to journalArticleAcademicpeer-review

    39 Citations (Scopus)

    Abstract

    Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.
    Original languageEnglish
    Pages (from-to)107-111
    JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
    Volume66
    DOIs
    Publication statusPublished - 2001

    Fingerprint Dive into the research topics of 'In Vitro conservation of enset under slow growth conditions'. Together they form a unique fingerprint.

    Cite this