Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 107-111 |
| Journal | Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants |
| Volume | 66 |
| DOIs | |
| Publication status | Published - 2001 |
Fingerprint
Cite this
}
In Vitro conservation of enset under slow growth conditions. / Negash, A.; Krens, F.A.; Schaart, J.G.; Visser, B.
In: Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants, Vol. 66, 2001, p. 107-111.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - In Vitro conservation of enset under slow growth conditions
AU - Negash, A.
AU - Krens, F.A.
AU - Schaart, J.G.
AU - Visser, B.
PY - 2001
Y1 - 2001
N2 - Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.
AB - Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.
U2 - 10.1023/A:1010647905508
DO - 10.1023/A:1010647905508
M3 - Article
VL - 66
SP - 107
EP - 111
JO - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
JF - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
SN - 0167-6857
ER -