In Vitro conservation of enset under slow growth conditions

A. Negash, F.A. Krens, J.G. Schaart, B. Visser

    Research output: Contribution to journalArticleAcademicpeer-review

    37 Citations (Scopus)

    Abstract

    Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.
    Original languageEnglish
    Pages (from-to)107-111
    JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
    Volume66
    DOIs
    Publication statusPublished - 2001

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    mannitol
    shoots
    Ethiopia
    in vitro culture
    growth retardation
    culture media
    clones
    greenhouses
    genetic variation
    crops
    temperature

    Cite this

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    title = "In Vitro conservation of enset under slow growth conditions",
    abstract = "Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2{\%} as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.",
    author = "A. Negash and F.A. Krens and J.G. Schaart and B. Visser",
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    In Vitro conservation of enset under slow growth conditions. / Negash, A.; Krens, F.A.; Schaart, J.G.; Visser, B.

    In: Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants, Vol. 66, 2001, p. 107-111.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - In Vitro conservation of enset under slow growth conditions

    AU - Negash, A.

    AU - Krens, F.A.

    AU - Schaart, J.G.

    AU - Visser, B.

    PY - 2001

    Y1 - 2001

    N2 - Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.

    AB - Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.

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    DO - 10.1023/A:1010647905508

    M3 - Article

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    JF - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

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