In Vitro conservation of enset under slow growth conditions

A. Negash; F.A. Krens; J.G. Schaart; B. Visser

doi:10.1023/A:1010647905508

In Vitro conservation of enset under slow growth conditions

A. Negash, F.A. Krens, J.G. Schaart, B. Visser

Research output: Contribution to journal › Article › Academic › peer-review

39 Citations (Scopus)

Abstract

Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.

Original language	English
Pages (from-to)	107-111
Journal	Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
Volume	66
DOIs	https://doi.org/10.1023/A:1010647905508
Publication status	Published - 2001

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title = "In Vitro conservation of enset under slow growth conditions",

abstract = "Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.",

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AU - Negash, A.

AU - Krens, F.A.

AU - Schaart, J.G.

AU - Visser, B.

PY - 2001

Y1 - 2001

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AB - Studies on in vitro storage of enset under slow-growth conditions were carried out to develop an efficient protocol for conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using three enset clones collected from southwestern Ethiopia. In vitro cultures could be effectively maintained for 6 months at 15 °C and 18 °C on MS medium supplemented with 10 M BAP, in the presence of mannitol at concentrations of 0, 1 or 2% as a growth retardant. Shoots were subsequently recovered and multiplied on MS medium supplemented with 10 and 20 M BAP at 25 °C and rooted shoots were successfully transferred to the greenhouse. Incubation at the lower temperature (15 °C) and the presence of mannitol in the culture medium had a significantly positive effect on maintenance, measured by the number of recovered shoots after storage.

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DO - 10.1023/A:1010647905508

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EP - 111

JO - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

JF - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

SN - 0167-6857

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