In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

B. Menin; A. Moglia; C. Comino; S. Lanteri; T.W.J.M. van Herpen; M.J. Beekwilder

doi:10.17660/ActaHortic.2012.961.34

In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

B. Menin, A. Moglia, C. Comino, S. Lanteri, T.W.J.M. van Herpen, M.J. Beekwilder

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

4 Citations (Scopus)

Abstract

Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type ‘Romanesco’; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter. After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.

Original language	English
Title of host publication	Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011
Editors	D. Geelen
Place of Publication	Leuven
Publisher	ISHS
Pages	267-271
Volume	961
DOIs	https://doi.org/10.17660/ActaHortic.2012.961.34
Publication status	Published - 2012
Event	VIIth international symposium on in vitro culture and horticultural breeding - Duration: 18 Sept 2011 → 22 Sept 2011

Conference

Conference	VIIth international symposium on in vitro culture and horticultural breeding
Period	18/09/11 → 22/09/11

Keywords

Agrobacterium tumefaciens
Callus culture
Genetic transformation
Globe artichoke

Access to Document

10.17660/ActaHortic.2012.961.34

Cite this

Menin, B., Moglia, A., Comino, C., Lanteri, S., van Herpen, T. W. J. M., & Beekwilder, M. J. (2012). In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke. In D. Geelen (Ed.), Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011 (Vol. 961, pp. 267-271). ISHS. https://doi.org/10.17660/ActaHortic.2012.961.34

@inproceedings{d28325b312c84df985bdbacba8ab888a,

title = "In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke",

abstract = "Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type {\textquoteleft}Romanesco{\textquoteright}; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter. After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.",

keywords = "Agrobacterium tumefaciens, Callus culture, Genetic transformation, Globe artichoke",

author = "B. Menin and A. Moglia and C. Comino and S. Lanteri and {van Herpen}, T.W.J.M. and M.J. Beekwilder",

year = "2012",

doi = "10.17660/ActaHortic.2012.961.34",

language = "English",

volume = "961",

pages = "267--271",

editor = "D. Geelen",

booktitle = "Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011",

publisher = "ISHS",

address = "Belgium",

note = "VIIth international symposium on in vitro culture and horticultural breeding ; Conference date: 18-09-2011 Through 22-09-2011",

}

Menin, B, Moglia, A, Comino, C, Lanteri, S, van Herpen, TWJM & Beekwilder, MJ 2012, In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke. in D Geelen (ed.), Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011. vol. 961, ISHS, Leuven, pp. 267-271, VIIth international symposium on in vitro culture and horticultural breeding, 18/09/11. https://doi.org/10.17660/ActaHortic.2012.961.34

In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke. / Menin, B.; Moglia, A.; Comino, C. et al.
Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011. ed. / D. Geelen. Vol. 961 Leuven: ISHS, 2012. p. 267-271.

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

TY - GEN

T1 - In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

AU - Menin, B.

AU - Moglia, A.

AU - Comino, C.

AU - Lanteri, S.

AU - van Herpen, T.W.J.M.

AU - Beekwilder, M.J.

PY - 2012

Y1 - 2012

N2 - Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type ‘Romanesco’; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter. After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.

AB - Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type ‘Romanesco’; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter. After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.

KW - Agrobacterium tumefaciens

KW - Callus culture

KW - Genetic transformation

KW - Globe artichoke

U2 - 10.17660/ActaHortic.2012.961.34

DO - 10.17660/ActaHortic.2012.961.34

M3 - Conference paper

VL - 961

SP - 267

EP - 271

BT - Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011

A2 - Geelen, D.

PB - ISHS

CY - Leuven

T2 - VIIth international symposium on in vitro culture and horticultural breeding

Y2 - 18 September 2011 through 22 September 2011

ER -

Menin B, Moglia A, Comino C, Lanteri S, van Herpen TWJM, Beekwilder MJ. In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke. In Geelen D, editor, Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011. Vol. 961. Leuven: ISHS. 2012. p. 267-271 doi: 10.17660/ActaHortic.2012.961.34

In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

Abstract

Conference

Keywords

Access to Document

Fingerprint

Cite this