In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

B. Menin, A. Moglia, C. Comino, S. Lanteri, T.W.J.M. van Herpen, M.J. Beekwilder

Research output: Chapter in Book/Report/Conference proceedingConference paper

3 Citations (Scopus)

Abstract

Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We developed an efficient protocol for callus induction from leaf explants of three globe artichoke genotypes of the varietal type ‘Romanesco’; after just one week culture callus formed with an efficiency close to 100%. Leaf explants were transformed with Agrobacterium tumefaciens Agl0 01-124 strain, harboring the binary vector pCAMBIA 2301 with the gene marker GUS, under the control of CaMV 35S promoter. After two weeks, about 30% of the calli obtained from infected leaf explants were positive to GUS assay.
Original languageEnglish
Title of host publicationProceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011
EditorsD. Geelen
Place of PublicationLeuven
PublisherISHS
Pages267-271
Volume961
DOIs
Publication statusPublished - 2012
EventVIIth international symposium on in vitro culture and horticultural breeding -
Duration: 18 Sep 201122 Sep 2011

Conference

ConferenceVIIth international symposium on in vitro culture and horticultural breeding
Period18/09/1122/09/11

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  • Cite this

    Menin, B., Moglia, A., Comino, C., Lanteri, S., van Herpen, T. W. J. M., & Beekwilder, M. J. (2012). In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke. In D. Geelen (Ed.), Proceedings of the VIIth international symposium on in vitro culture and horticultural breeding : Ghent Belgium September 18-22, 2011 (Vol. 961, pp. 267-271). Leuven: ISHS. https://doi.org/10.17660/ActaHortic.2012.961.34