TY - JOUR
T1 - In vitro bioassays to evaluate beneficial and adverse health effects of botanicals
T2 - Promises and pitfalls
AU - Prinsloo, Gerhard
AU - Papadi, Georgia
AU - Hiben, Mebrahtom G.
AU - de Haan, Laura
AU - Louisse, Jochem
AU - Beekmann, Karsten
AU - Vervoort, Jacques
AU - Rietjens, Ivonne M.C.M.
PY - 2017
Y1 - 2017
N2 - This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated. In vitro assays are widely and effectively used to test the efficacy and adverse effects of botanicals and botanical preparations. The pitfalls of these assays are however often incorrectly and inaccurately taken into account hampering adequate extrapolation to in vivo situations often resulting in false interpretations. These pitfalls as well as strategies to overcome them are discussed.
AB - This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated. In vitro assays are widely and effectively used to test the efficacy and adverse effects of botanicals and botanical preparations. The pitfalls of these assays are however often incorrectly and inaccurately taken into account hampering adequate extrapolation to in vivo situations often resulting in false interpretations. These pitfalls as well as strategies to overcome them are discussed.
U2 - 10.1016/j.drudis.2017.05.002
DO - 10.1016/j.drudis.2017.05.002
M3 - Article
AN - SCOPUS:85020621191
SN - 1359-6446
VL - 22
SP - 1187
EP - 1200
JO - Drug Discovery Today
JF - Drug Discovery Today
IS - 8
ER -