In vitro bioassays to evaluate beneficial and adverse health effects of botanicals: Promises and pitfalls

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Abstract

This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated. In vitro assays are widely and effectively used to test the efficacy and adverse effects of botanicals and botanical preparations. The pitfalls of these assays are however often incorrectly and inaccurately taken into account hampering adequate extrapolation to in vivo situations often resulting in false interpretations. These pitfalls as well as strategies to overcome them are discussed.
Original languageEnglish
Pages (from-to)1187-1200
JournalDrug Discovery Today
Volume22
Issue number8
DOIs
Publication statusPublished - 2017

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Biological Assay
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In Vitro Techniques

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title = "In vitro bioassays to evaluate beneficial and adverse health effects of botanicals: Promises and pitfalls",
abstract = "This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated. In vitro assays are widely and effectively used to test the efficacy and adverse effects of botanicals and botanical preparations. The pitfalls of these assays are however often incorrectly and inaccurately taken into account hampering adequate extrapolation to in vivo situations often resulting in false interpretations. These pitfalls as well as strategies to overcome them are discussed.",
author = "Gerhard Prinsloo and Georgia Papadi and Hiben, {Mebrahtom G.} and {de Haan}, Laura and Jochem Louisse and Karsten Beekmann and Jacques Vervoort and Rietjens, {Ivonne M.C.M.}",
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TY - JOUR

T1 - In vitro bioassays to evaluate beneficial and adverse health effects of botanicals

T2 - Promises and pitfalls

AU - Prinsloo, Gerhard

AU - Papadi, Georgia

AU - Hiben, Mebrahtom G.

AU - de Haan, Laura

AU - Louisse, Jochem

AU - Beekmann, Karsten

AU - Vervoort, Jacques

AU - Rietjens, Ivonne M.C.M.

PY - 2017

Y1 - 2017

N2 - This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated. In vitro assays are widely and effectively used to test the efficacy and adverse effects of botanicals and botanical preparations. The pitfalls of these assays are however often incorrectly and inaccurately taken into account hampering adequate extrapolation to in vivo situations often resulting in false interpretations. These pitfalls as well as strategies to overcome them are discussed.

AB - This review provides an update on the promises and pitfalls when using in vitro bioassays to evaluate beneficial and adverse health effects of botanicals and botanical preparations. Important issues addressed in the paper are: (i) the type of assays and biological effects available; (ii) false-positives, false-negatives and confounding factors; (iii) matrix and combination effects; (iv) extrapolation of in vitro data to the in vivo situation; (v) when (not) to use bioassays; and (vi) identification of active constituents. It is concluded that in vitro bioassays provide models to detect beneficial as well as adverse activities, but that linking these observations to individual ingredients and extrapolations to the in vivo situation is more complicated than generally anticipated. In vitro assays are widely and effectively used to test the efficacy and adverse effects of botanicals and botanical preparations. The pitfalls of these assays are however often incorrectly and inaccurately taken into account hampering adequate extrapolation to in vivo situations often resulting in false interpretations. These pitfalls as well as strategies to overcome them are discussed.

U2 - 10.1016/j.drudis.2017.05.002

DO - 10.1016/j.drudis.2017.05.002

M3 - Article

VL - 22

SP - 1187

EP - 1200

JO - Drug Discovery Today

JF - Drug Discovery Today

SN - 1359-6446

IS - 8

ER -