Many characterized fungal effector proteins are small secreted proteins. Effectors are defined as those proteins that alter host cell structure and/or function by facilitating pathogen infection. The identification of effectors by molecular and cell biology techniques is a difficult task. However, with the availability of whole-genome sequences, these proteins can now be predicted in silico. Here, we describe in detail how to identify and characterize effectors from a defined fungal proteome using in silico techniques.
|Title of host publication||Plant Fungal Pathogens: Methods and Protocols|
|Editors||M.D. Bolton, B.P.H.J Thomma|
|Place of Publication||New York, USA|
|Number of pages||648|
|Publication status||Published - 2012|
|Name||Methods in Molecular Biology|
|Publisher||Humana Press, c/o Springer Science+Business Media|