In silico identification and characterization of effector catalogs

R. de Jonge

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

8 Citations (Scopus)

Abstract

Many characterized fungal effector proteins are small secreted proteins. Effectors are defined as those proteins that alter host cell structure and/or function by facilitating pathogen infection. The identification of effectors by molecular and cell biology techniques is a difficult task. However, with the availability of whole-genome sequences, these proteins can now be predicted in silico. Here, we describe in detail how to identify and characterize effectors from a defined fungal proteome using in silico techniques.
Original languageEnglish
Title of host publicationPlant Fungal Pathogens: Methods and Protocols
EditorsM.D. Bolton, B.P.H.J Thomma
Place of PublicationNew York, USA
Pages415-425
Number of pages648
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media
Number835

Keywords

  • Effector
  • GO Terms
  • Host
  • Interaction
  • InterProScan
  • Pathogen
  • PHI-base
  • Secretome
  • SignalP
  • WoLF PSORT

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