Imputation of 3 million SNPs in the Arabidopsis regional mapping population

Bader Arouisse, Arthur Korte, Fred van Eeuwijk, Willem Kruijer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)


Natural variation has become a prime resource to identify genetic variants that contribute to phenotypic variation. The regional mapping (RegMap) population is one of the most important populations for studying natural variation in Arabidopsis thaliana, and has been used in a large number of association studies and in studies on climatic adaptation. However, only 413 RegMap accessions have been completely sequenced, as part of the 1001 Genomes (1001G) Project, while the remaining 894 accessions have only been genotyped with the Affymetrix 250k chip. As a consequence, most association studies involving the RegMap are either restricted to the sequenced accessions, reducing power, or rely on a limited set of SNPs. Here we impute millions of SNPs to the 894 accessions that are exclusive to the RegMap, using the 1135 accessions of the 1001G Project as the reference panel. We assess imputation accuracy using a novel cross-validation scheme, which we show provides a more reliable measure of accuracy than existing methods. After filtering out low accuracy SNPs, we obtain high-quality genotypic information for 2029 accessions and 3 million markers. To illustrate the benefits of these imputed data, we reconducted genome-wide association studies on five stress-related traits and could identify novel candidate genes.

Original languageEnglish
Pages (from-to)872-882
JournalPlant Journal
Issue number4
Early online date19 Dec 2019
Publication statusPublished - May 2020


  • 1001 Genomes project
  • Arabidopsis thaliana
  • genome-wide association study
  • imputation accuracy
  • regional mapping


Dive into the research topics of 'Imputation of 3 million SNPs in the Arabidopsis regional mapping population'. Together they form a unique fingerprint.

Cite this