Improving scFv antibody expression levels in the plant cytosol.

A. Schouten, J. Roosien, J.M. de Boer, A. Wilmink, M.N. rosso, D. Bosch, W.J. Stiekema, F.J. gommers, J. Bakker, A. Schots

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Expression of single-chain antibody fragments (scFvs) in the plant cytosol is often cumbersome. It was unexpectedly shown that addition at the C-terminus of the ER retention signal KDEL resulted in significantly improved expression levels. In this report the cytosolic location of the scFv-CK was confirmed, excluding possible mistranslocation to other subcellular compartments. It was shown that expression of several other scFvs was also improved in tobacco protoplasts. In addition expression was improved in transgenic potato. Changing from KDEL to KDEI did not affect the enhanced protein expression level. Addition of the KDEL motif is a simple and straightforward tool to stabilize in plants cytosolic expression of many scFvs.
Original languageEnglish
Pages (from-to)235-241
JournalFEBS Letters
Volume415
DOIs
Publication statusPublished - 1997

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    Schouten, A., Roosien, J., de Boer, J. M., Wilmink, A., rosso, M. N., Bosch, D., ... Schots, A. (1997). Improving scFv antibody expression levels in the plant cytosol. FEBS Letters, 415, 235-241. https://doi.org/10.1016/S0014-5793(97)01129-0