Improving Jatropha curcas seed protein recovery by using counter current multistage extraction

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Abstract

Jatropha curcas seed press cake contains 23 wt% proteins (dry basis). Due to the toxic compounds in Jatropha, we will use the proteins for non-food applications. Related to non-food applications, an efficient protein extraction to obtain a high protein recovery and high protein concentration with good protein functional properties is required. To achieve this, we conducted protein extractions at room temperature by using different solvents at various solvent to solid ratios and performed multistage counter current extraction to improve protein recovery and protein concentration. We obtained the highest protein recovery of 82% by using 0.055 M NaOH at solvent to solid ratio of 10 g/g after four-stage counter current extraction. By using 0.055 M NaOH extraction at lower solvent to solid ratio of 4 g/g, protein recovery was 35% after one-stage extraction and improved up to 71% with a high protein concentration of 63 mg/mL after four-stage counter current extraction. The highest amount of protein was precipitated from crude extracts at pH 4–5.5 and resulted on precipitate with protein content of 70%. The multistage counter current extraction did not influence Jatropha seed protein molecular weight distributions, which were within the range of 3–98 kDa
LanguageEnglish
Pages16-23
JournalBiochemical Engineering Journal
Volume50
Issue number1-2
DOIs
Publication statusPublished - 2010

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Jatropha
Seed
Seeds
Proteins
Recovery
Poisons

Keywords

  • antinutritional factors
  • products
  • oil
  • plant

Cite this

@article{7c231e922d9646fc9ce244be4cbdf9a2,
title = "Improving Jatropha curcas seed protein recovery by using counter current multistage extraction",
abstract = "Jatropha curcas seed press cake contains 23 wt{\%} proteins (dry basis). Due to the toxic compounds in Jatropha, we will use the proteins for non-food applications. Related to non-food applications, an efficient protein extraction to obtain a high protein recovery and high protein concentration with good protein functional properties is required. To achieve this, we conducted protein extractions at room temperature by using different solvents at various solvent to solid ratios and performed multistage counter current extraction to improve protein recovery and protein concentration. We obtained the highest protein recovery of 82{\%} by using 0.055 M NaOH at solvent to solid ratio of 10 g/g after four-stage counter current extraction. By using 0.055 M NaOH extraction at lower solvent to solid ratio of 4 g/g, protein recovery was 35{\%} after one-stage extraction and improved up to 71{\%} with a high protein concentration of 63 mg/mL after four-stage counter current extraction. The highest amount of protein was precipitated from crude extracts at pH 4–5.5 and resulted on precipitate with protein content of 70{\%}. The multistage counter current extraction did not influence Jatropha seed protein molecular weight distributions, which were within the range of 3–98 kDa",
keywords = "antinutritional factors, products, oil, plant",
author = "D. Lestari and W.J. Mulder and J.P.M. Sanders",
year = "2010",
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language = "English",
volume = "50",
pages = "16--23",
journal = "Biochemical Engineering Journal",
issn = "1369-703X",
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Improving Jatropha curcas seed protein recovery by using counter current multistage extraction. / Lestari, D.; Mulder, W.J.; Sanders, J.P.M.

In: Biochemical Engineering Journal, Vol. 50, No. 1-2, 2010, p. 16-23.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Improving Jatropha curcas seed protein recovery by using counter current multistage extraction

AU - Lestari, D.

AU - Mulder, W.J.

AU - Sanders, J.P.M.

PY - 2010

Y1 - 2010

N2 - Jatropha curcas seed press cake contains 23 wt% proteins (dry basis). Due to the toxic compounds in Jatropha, we will use the proteins for non-food applications. Related to non-food applications, an efficient protein extraction to obtain a high protein recovery and high protein concentration with good protein functional properties is required. To achieve this, we conducted protein extractions at room temperature by using different solvents at various solvent to solid ratios and performed multistage counter current extraction to improve protein recovery and protein concentration. We obtained the highest protein recovery of 82% by using 0.055 M NaOH at solvent to solid ratio of 10 g/g after four-stage counter current extraction. By using 0.055 M NaOH extraction at lower solvent to solid ratio of 4 g/g, protein recovery was 35% after one-stage extraction and improved up to 71% with a high protein concentration of 63 mg/mL after four-stage counter current extraction. The highest amount of protein was precipitated from crude extracts at pH 4–5.5 and resulted on precipitate with protein content of 70%. The multistage counter current extraction did not influence Jatropha seed protein molecular weight distributions, which were within the range of 3–98 kDa

AB - Jatropha curcas seed press cake contains 23 wt% proteins (dry basis). Due to the toxic compounds in Jatropha, we will use the proteins for non-food applications. Related to non-food applications, an efficient protein extraction to obtain a high protein recovery and high protein concentration with good protein functional properties is required. To achieve this, we conducted protein extractions at room temperature by using different solvents at various solvent to solid ratios and performed multistage counter current extraction to improve protein recovery and protein concentration. We obtained the highest protein recovery of 82% by using 0.055 M NaOH at solvent to solid ratio of 10 g/g after four-stage counter current extraction. By using 0.055 M NaOH extraction at lower solvent to solid ratio of 4 g/g, protein recovery was 35% after one-stage extraction and improved up to 71% with a high protein concentration of 63 mg/mL after four-stage counter current extraction. The highest amount of protein was precipitated from crude extracts at pH 4–5.5 and resulted on precipitate with protein content of 70%. The multistage counter current extraction did not influence Jatropha seed protein molecular weight distributions, which were within the range of 3–98 kDa

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KW - products

KW - oil

KW - plant

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