Improved production of medium-chain-length Polyhydroxyalkanotes in glucose-based fed-batch cultivations of metabolically engineered Pseudomonas putida strains

I. Poblete-Castro, A.L. Rodriguez, M.C. Lam, W. Kessler

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

One of the major challenges in metabolic engineering for enhanced synthesis of value-added chemicals is to design and develop new strains which can be translated into well-controlled fermentation processes using bioreactors. The aim of this study was to assess the influence of various fed-batch strategies in the performance of metabolically-engineered Pseudomonas putida strains, ¿gcd and ¿gcd-pgl, for improving production of medium-chain-length poly-hydroxyalkanoates (mcl-PHAs) using glucose as the only carbon source. First we developed a fed-batch process which comprised an initial phase of biomass accumulation based on an exponential feeding carbon-limited strategy. For the mcl-PHA accumulation stage, three induction techniques were tested under nitrogen limitation. The substrate-pulse feeding was more efficient than the constant-feeding approach to promote the accumulation of the desirable product. Nonetheless, the most efficient approach for maximum PHA synthesis was the application of a dissolved-oxygen-stat feeding strategy (DO-stat), where P. putida ¿gcd mutant strain showed a final PHA content and specific PHA productivity of 67%, and 0.83 [g•L-1•h-1], respectively. To our knowledge this mcl-PHA titer is the highest value that has been ever reported using glucose as the solely carbon and energy source. Our results also highlighted the effect of different fed-batch strategies upon the extent of realization of the intended metabolic modification of the mutant strains
Original languageEnglish
Pages (from-to)59-69
JournalJournal of Microbiology and Biotechnology
Volume24
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • cell-density cultivation
  • mcl-pha
  • catabolite repression
  • unsaturated monomers
  • nonanoic acid
  • biosynthesis
  • kt2442
  • poly(3-hydroxyalkanoates)
  • poly(hydroxyalkanoates)
  • accumulation

Fingerprint Dive into the research topics of 'Improved production of medium-chain-length Polyhydroxyalkanotes in glucose-based fed-batch cultivations of metabolically engineered Pseudomonas putida strains'. Together they form a unique fingerprint.

  • Cite this