Improved postthaw viability and in vitro functionality of peripheral blood hematopoietic progenitor cells after cryopreservation with a theoretically optimized freezing curve

M.R. Tijssen, H. Woelders, A. de Vries - van Rossen, C. van der Schoot, C. Voermans, J.W.M. Lagerberg

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

The freezing curve currently used for the cryopreservation of peripheral blood stem cell transplants (PBSCTs) has been determined empirically. Although the use of cryopreserved PBSCTs is successful and usually leads to rapid hematopoietic recovery, the freeze-thawing process is known to induce a significant degree of cell death. Furthermore, the infusion of dimethyl sulfoxide (DMSO), used to protect the cells against damage induced by freezing, can cause morbidity. Therefore, optimizing the current cryopreservation protocol (with 10% DMSO and a slow linear cooling curve) with theoretically optimized freezing curves and a lower DMSO concentration might improve the recovery after transplantation
Original languageEnglish
Pages (from-to)893-901
JournalTransfusion
Volume48
Issue number5
DOIs
Publication statusPublished - 2008

Keywords

  • cd34(+) cells
  • clinical toxicity
  • stem-cells
  • apoptosis
  • permeability
  • infusion
  • grafts
  • marrow
  • death

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