TY - JOUR
T1 - Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry
AU - Bañuls, Maria Jose
AU - Jiménez-Meneses, Pilar
AU - Meyer, Albert
AU - Vasseur, Jean Jacques
AU - Morvan, François
AU - Escorihuela, Jorge
AU - Puchades, Rosa
AU - Maquieira, Ángel
PY - 2017/2/15
Y1 - 2017/2/15
N2 - Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.
AB - Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.
U2 - 10.1021/acs.bioconjchem.6b00624
DO - 10.1021/acs.bioconjchem.6b00624
M3 - Article
C2 - 28042940
AN - SCOPUS:85013128273
SN - 1043-1802
VL - 28
SP - 496
EP - 506
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 2
ER -