Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry

Maria Jose Bañuls, Pilar Jiménez-Meneses, Albert Meyer, Jean Jacques Vasseur, François Morvan*, Jorge Escorihuela, Rosa Puchades, Ángel Maquieira

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.

Original languageEnglish
Pages (from-to)496-506
Number of pages11
JournalBioconjugate Chemistry
Volume28
Issue number2
DOIs
Publication statusPublished - 15 Feb 2017

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    Bañuls, M. J., Jiménez-Meneses, P., Meyer, A., Vasseur, J. J., Morvan, F., Escorihuela, J., Puchades, R., & Maquieira, Á. (2017). Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry. Bioconjugate Chemistry, 28(2), 496-506. https://doi.org/10.1021/acs.bioconjchem.6b00624