Projects per year
This work aimed to select a Tisochrysis lutea phenotype with higher biomass and fucoxanthin productivities using fluorescence-activated cell sorting (FACS). A novel phenotype was obtained after 2 rounds of selection, based on high-fucoxanthin fluorescence. The resulting phenotype forms cell aggregates, has no flagella, and was stable after 15 months. Optimal temperature (30 °C) and light (300 µmol m−2 s−1) were obtained at laboratory scale, identical to the original strain. The biomass productivity was higher than the original strain: 1.9× at laboratory scale (0.4 L), and 4.5× under outdoor conditions (190 L). Moreover, compared to the original strain, the productivity of fucoxanthin increased 1.6–3.1× and docosahexaenoic acid 1.5–1.9×. These are the highest ever reported outdoor productivities, obtained with a robust new phenotype from a T. lutea monoculture isolated with FACS without genetic manipulation. The resulting phenotype shows high potential for industrial production.
- Fluorescence-activated cell sorting (FACS)
- High productivity
- Robust new phenotype
- Tisochrysis lutea
FingerprintDive into the research topics of 'Improved fucoxanthin and docosahexaenoic acid productivities of a sorted self-settling Tisochrysis lutea phenotype at pilot scale'. Together they form a unique fingerprint.
- 1 Finished
MAGNIFICENT: Microalgae As a Green source for Nutritional Ingredients for Food/Feed and Ingredients for Cosmetics by cost-Effective New Technologies
1/06/17 → 30/11/21
Project: EU research project