Improved CRISPR-Cpf1 genome editing tool 

J. van der Oost (Inventor), P. van Baarlen (Inventor), Rogier Louwen (Inventor)

Research output: PatentOther research output

Abstract

The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.
Original languageEnglish
Patent numberWO2017155407
Priority date11/03/16
Publication statusPublished - 14 Sep 2017

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Clustered Regularly Interspaced Short Palindromic Repeats
Guide RNA
Proteins
Gene Editing

Cite this

van der Oost, J., van Baarlen, P., & Louwen, R. (2017). Improved CRISPR-Cpf1 genome editing tool . (Patent No. WO2017155407).
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Improved CRISPR-Cpf1 genome editing tool . / van der Oost, J. (Inventor); van Baarlen, P. (Inventor); Louwen, Rogier (Inventor).

Patent No.: WO2017155407.

Research output: PatentOther research output

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AB - The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.

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van der Oost J, van Baarlen P, Louwen R, inventors. Improved CRISPR-Cpf1 genome editing tool . WO2017155407. 2017 Sep 14.