Improved CRISPR-Cpf1 genome editing tool 

J. van der Oost (Inventor), P. van Baarlen (Inventor), Rogier Louwen (Inventor)

Research output: Patent › Other research output

Abstract

The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.
Original languageEnglish
Patent numberWO2017155407
Priority date11/03/16
Publication statusPublished - 14 Sep 2017

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Guide RNA
Proteins
Gene Editing

Cite this

van der Oost, J., van Baarlen, P., & Louwen, R. (2017). Improved CRISPR-Cpf1 genome editing tool . (Patent No. WO2017155407).
van der Oost, J. (Inventor) ; van Baarlen, P. (Inventor) ; Louwen, Rogier (Inventor). / Improved CRISPR-Cpf1 genome editing tool . Patent No.: WO2017155407.
@misc{5c6e74fa788a41718474d15812c72435,
title = "Improved CRISPR-Cpf1 genome editing tool ",
abstract = "The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.",
author = "{van der Oost}, J. and {van Baarlen}, P. and Rogier Louwen",
year = "2017",
month = "9",
day = "14",
language = "English",
type = "Patent",
note = "WO2017155407",

}

van der Oost, J, van Baarlen, P & Louwen, R 2017, Improved CRISPR-Cpf1 genome editing tool , Patent No. WO2017155407.

Improved CRISPR-Cpf1 genome editing tool . / van der Oost, J. (Inventor); van Baarlen, P. (Inventor); Louwen, Rogier (Inventor).

Patent No.: WO2017155407.

Research output: Patent › Other research output

TY - PAT

T1 - Improved CRISPR-Cpf1 genome editing tool 

AU - van der Oost, J.

AU - van Baarlen, P.

AU - Louwen, Rogier

PY - 2017/9/14

Y1 - 2017/9/14

N2 - The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.

AB - The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.

M3 - Patent

M1 - WO2017155407

ER -

van der Oost J, van Baarlen P, Louwen R, inventors. Improved CRISPR-Cpf1 genome editing tool . WO2017155407. 2017 Sep 14.

Access to Document