Abstract
The invention relates to a Cpf1-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cpf1 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.
Original language | English |
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Patent number | WO2017155407 |
Priority date | 11/03/16 |
Publication status | Published - 14 Sep 2017 |