Improved CRISPR-Cas9 genome editing tool

Rogier Louwen (Inventor), J. van der Oost (Inventor)

Research output: PatentOther research output

Abstract

The invention relates to a Cas-based, preferably Cas9-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cas9 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.
Original languageEnglish
Patent numberWO2017155408
Priority date11/03/16
Publication statusPublished - 14 Sep 2017

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Clustered Regularly Interspaced Short Palindromic Repeats
Guide RNA
Proteins
Gene Editing

Cite this

Louwen, R., & van der Oost, J. (2017). Improved CRISPR-Cas9 genome editing tool. (Patent No. WO2017155408).
Louwen, Rogier (Inventor) ; van der Oost, J. (Inventor). / Improved CRISPR-Cas9 genome editing tool. Patent No.: WO2017155408.
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Louwen, R & van der Oost, J 2017, Improved CRISPR-Cas9 genome editing tool, Patent No. WO2017155408.

Improved CRISPR-Cas9 genome editing tool. / Louwen, Rogier (Inventor); van der Oost, J. (Inventor).

Patent No.: WO2017155408.

Research output: PatentOther research output

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Louwen R, van der Oost J, inventors. Improved CRISPR-Cas9 genome editing tool. WO2017155408. 2017 Sep 14.