Improved CRISPR-Cas9 genome editing tool

Rogier Louwen (Inventor), J. van der Oost (Inventor)

Research output: PatentOther research output


The invention relates to a Cas-based, preferably Cas9-based nuclease complex, wherein the guide RNA sequence is irreversibly crosslinked to the Cas9 protein. The cross-link may be a covalent binding or a non-covalent binding. Such a complex may be used in delivering constructs to a cell that are capable of gene-editing. Use of this cross-linked complex will result in less off-targeting.
Original languageEnglish
Patent numberWO2017155408
Priority date11/03/16
Publication statusPublished - 14 Sep 2017

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