Impaired β-adrenergically mediated lipolysis in skeletal muscle of obese subjects

E.E. Blaak*, S.L. Schiffelers, W.H. Saris, M. Mensink, M.E. Kooi

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Aims/hypothesis. The aim of this study was to investigate whether the β2-adrenergically mediated increase in interstitial glycerol concentrations (used as an indicator of local lipolysis) was impaired in the skeletal muscle (the gastrocnemius muscle) of obese subjects compared with in that of lean subjects, and whether any differences in muscle lipolysis were related to differences in intramyocellular muscle triglyceride content. Methods. A microdialysis experiment was performed in the gastrocnemius muscle of eight lean and eight obese men (body fat 22.1±1.6% vs 32.7±1.6% respectively). After determining baseline extracellular glycerol concentrations, the probe was perfused with increasing concentrations of the β2-agonist, salbutamol (doses of 1, 10 and 100 μmol/l for 45-min periods). Local blood flow was determined using the ethanol dilution technique. Intramyocellular lipid content was determined using 1H-magnetic resonance spectroscopy. Results. Compared with that in lean subjects, the β2-adrenergically mediated increase in glycerol concentrations (absolute and percentage change) was blunted in obese subjects (at 100 μmol/l of salbutamol, percentage change 12.0± 12% vs 48±12%, p<0.05). The decrease in ethanol out : in ratio was less pronounced in the obese individuals (p<0.05), indicating a diminished increase in local muscle blood flow. Intramyocellular lipid content was comparable in both groups. Conclusions/interpretation. The capacity to increase skeletal interstitial glycerol concentrations during direct β2-adrenergic stimulation is impaired in obese subjects with normal intramyocellular concentrations, suggesting that this may be an early event the process of triglyceride accumulation.

Original languageEnglish
Pages (from-to)1462-1468
Number of pages7
JournalDiabetologia
Volume47
Issue number8
DOIs
Publication statusPublished - 1 Aug 2004
Externally publishedYes

Keywords

  • β-adrenergic stimulation
  • Intramyocellular lipids
  • Lipolysis
  • Obesity
  • Skeletal muscle

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