Impact of sorbic acid on germination and outgrowth heterogeneity of Bacillus cereus ATCC 14579 spores

H.M.W. den Besten, C.C.J. van Melis, J.W. Sanders, M.N. Nierop Groot, T. Abee

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Population heterogeneity complicates the predictability of the outgrowth kinetics of individual spores. Flow cytometry sorting and monitoring of the germination and outgrowth of single dormant spores allowed the quantification of acid-induced spore population heterogeneity at pH 5.5 and in the presence of sorbic acid. This showed that germination efficiency was not a good predictor for heterogeneity in final outgrowth.
Original languageEnglish
Pages (from-to)8477-8480
JournalApplied and Environmental Microbiology
Volume78
Issue number23
DOIs
Publication statusPublished - 2012

Keywords

  • nonproteolytic clostridium-botulinum
  • heat-treatment
  • population heterogeneity
  • incubation-temperature
  • individual spores
  • single spores
  • atcc-14579
  • growth
  • times

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