Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity

A. Vrieze; C. Out; S. Fuentes Enriquez de Salamanca; L. Jonker; I. Reuling; R.S. Kootte; E. van Nood; F. Holleman; M. Knaapen; J.A. Romijn; M.R. Soeters; E.E. Blaak; G.M. Dallinga-Thie; D. Reijnders; M.T. Ackermans; M.J. Serlie; F.K. Knop; J.J. Holst; C.V. Ley; I.P. Kema; E.G. Zoetendal; W.M. de Vos; J.B. Hoekstra; E.S. Stroes; A.K. Groen; M. Nieuwdorp

doi:10.1016/j.jhep.2013.11.034

Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity

A. Vrieze, C. Out, S. Fuentes Enriquez de Salamanca, L. Jonker, I. Reuling, R.S. Kootte, E. van Nood, F. Holleman, M. Knaapen, J.A. Romijn, M.R. Soeters, E.E. Blaak, G.M. Dallinga-Thie, D. Reijnders, M.T. Ackermans, M.J. Serlie, F.K. Knop, J.J. Holst, C.V. Ley, I.P. KemaE.G. Zoetendal, W.M. de Vos, J.B. Hoekstra, E.S. Stroes, A.K. Groen, M. Nieuwdorp

Research output: Contribution to journal › Article › Academic › peer-review

470 Citations (Scopus)

Abstract

BACKGROUND: Obesity has been associated with changes in the composition and function of the intestinal microbiota. Modulation of the microbiota by antibiotics also alters bile acid and glucose metabolism in mice. Hence, we hypothesized that short term administration of oral antibiotics in humans would affect fecal microbiota composition and subsequently bile acid and glucose metabolism. METHODS: In this single blinded randomized controlled trial, 20 male obese subjects with metabolic syndrome were randomized to 7 days of amoxicillin 500mg t.i.d. or 7 days of vancomycin 500mg t.i.d. At baseline and after 1 week of therapy, fecal microbiota composition (Human Intestinal Tract Chip phylogenetic microarray), fecal and plasma bile acid concentrations as well as insulin sensitivity (hyperinsulinemic euglycemic clamp using [6,6-2H2]-glucose tracer) were measured. RESULTS: Vancomycin reduced fecal microbial diversity with a decrease of gram-positive bacteria (mainly Firmicutes) and a compensatory increase in gram-negative bacteria (mainly Proteobacteria). Concomitantly, vancomycin decreased fecal secondary bile acids with a simultaneous postprandial increase in primary bile acids in plasma (p

Original language	English
Pages (from-to)	824-831
Journal	Journal of Hepatology
Volume	60
Issue number	4
DOIs	https://doi.org/10.1016/j.jhep.2013.11.034
Publication status	Published - 2014

Keywords

salt hydrolase activity
diet-induced obesity
intestinal microbiota
energy-expenditure
mice
resistance
adiposity
glucagon
capacity
humans

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jhep.2013.11.034

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Vrieze, A., Out, C., Fuentes Enriquez de Salamanca, S., Jonker, L., Reuling, I., Kootte, R. S., van Nood, E., Holleman, F., Knaapen, M., Romijn, J. A., Soeters, M. R., Blaak, E. E., Dallinga-Thie, G. M., Reijnders, D., Ackermans, M. T., Serlie, M. J., Knop, F. K., Holst, J. J., Ley, C. V., ... Nieuwdorp, M. (2014). Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity. Journal of Hepatology, 60(4), 824-831. https://doi.org/10.1016/j.jhep.2013.11.034

@article{d3633c3062ed41329f6ec8eb0329a0d8,

title = "Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity",

abstract = "BACKGROUND: Obesity has been associated with changes in the composition and function of the intestinal microbiota. Modulation of the microbiota by antibiotics also alters bile acid and glucose metabolism in mice. Hence, we hypothesized that short term administration of oral antibiotics in humans would affect fecal microbiota composition and subsequently bile acid and glucose metabolism. METHODS: In this single blinded randomized controlled trial, 20 male obese subjects with metabolic syndrome were randomized to 7 days of amoxicillin 500mg t.i.d. or 7 days of vancomycin 500mg t.i.d. At baseline and after 1 week of therapy, fecal microbiota composition (Human Intestinal Tract Chip phylogenetic microarray), fecal and plasma bile acid concentrations as well as insulin sensitivity (hyperinsulinemic euglycemic clamp using [6,6-2H2]-glucose tracer) were measured. RESULTS: Vancomycin reduced fecal microbial diversity with a decrease of gram-positive bacteria (mainly Firmicutes) and a compensatory increase in gram-negative bacteria (mainly Proteobacteria). Concomitantly, vancomycin decreased fecal secondary bile acids with a simultaneous postprandial increase in primary bile acids in plasma (p",

keywords = "salt hydrolase activity, diet-induced obesity, intestinal microbiota, energy-expenditure, mice, resistance, adiposity, glucagon, capacity, humans",

author = "A. Vrieze and C. Out and {Fuentes Enriquez de Salamanca}, S. and L. Jonker and I. Reuling and R.S. Kootte and {van Nood}, E. and F. Holleman and M. Knaapen and J.A. Romijn and M.R. Soeters and E.E. Blaak and G.M. Dallinga-Thie and D. Reijnders and M.T. Ackermans and M.J. Serlie and F.K. Knop and J.J. Holst and C.V. Ley and I.P. Kema and E.G. Zoetendal and {de Vos}, W.M. and J.B. Hoekstra and E.S. Stroes and A.K. Groen and M. Nieuwdorp",

year = "2014",

doi = "10.1016/j.jhep.2013.11.034",

language = "English",

volume = "60",

pages = "824--831",

journal = "Journal of Hepatology",

issn = "0168-8278",

publisher = "Elsevier",

number = "4",

}

Vrieze, A, Out, C, Fuentes Enriquez de Salamanca, S, Jonker, L, Reuling, I, Kootte, RS, van Nood, E, Holleman, F, Knaapen, M, Romijn, JA, Soeters, MR, Blaak, EE, Dallinga-Thie, GM, Reijnders, D, Ackermans, MT, Serlie, MJ, Knop, FK, Holst, JJ, Ley, CV, Kema, IP, Zoetendal, EG , de Vos, WM, Hoekstra, JB, Stroes, ES, Groen, AK & Nieuwdorp, M 2014, 'Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity', Journal of Hepatology, vol. 60, no. 4, pp. 824-831. https://doi.org/10.1016/j.jhep.2013.11.034

TY - JOUR

T1 - Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity

AU - Vrieze, A.

AU - Out, C.

AU - Fuentes Enriquez de Salamanca, S.

AU - Jonker, L.

AU - Reuling, I.

AU - Kootte, R.S.

AU - van Nood, E.

AU - Holleman, F.

AU - Knaapen, M.

AU - Romijn, J.A.

AU - Soeters, M.R.

AU - Blaak, E.E.

AU - Dallinga-Thie, G.M.

AU - Reijnders, D.

AU - Ackermans, M.T.

AU - Serlie, M.J.

AU - Knop, F.K.

AU - Holst, J.J.

AU - Ley, C.V.

AU - Kema, I.P.

AU - Zoetendal, E.G.

AU - de Vos, W.M.

AU - Hoekstra, J.B.

AU - Stroes, E.S.

AU - Groen, A.K.

AU - Nieuwdorp, M.

PY - 2014

Y1 - 2014

N2 - BACKGROUND: Obesity has been associated with changes in the composition and function of the intestinal microbiota. Modulation of the microbiota by antibiotics also alters bile acid and glucose metabolism in mice. Hence, we hypothesized that short term administration of oral antibiotics in humans would affect fecal microbiota composition and subsequently bile acid and glucose metabolism. METHODS: In this single blinded randomized controlled trial, 20 male obese subjects with metabolic syndrome were randomized to 7 days of amoxicillin 500mg t.i.d. or 7 days of vancomycin 500mg t.i.d. At baseline and after 1 week of therapy, fecal microbiota composition (Human Intestinal Tract Chip phylogenetic microarray), fecal and plasma bile acid concentrations as well as insulin sensitivity (hyperinsulinemic euglycemic clamp using [6,6-2H2]-glucose tracer) were measured. RESULTS: Vancomycin reduced fecal microbial diversity with a decrease of gram-positive bacteria (mainly Firmicutes) and a compensatory increase in gram-negative bacteria (mainly Proteobacteria). Concomitantly, vancomycin decreased fecal secondary bile acids with a simultaneous postprandial increase in primary bile acids in plasma (p

AB - BACKGROUND: Obesity has been associated with changes in the composition and function of the intestinal microbiota. Modulation of the microbiota by antibiotics also alters bile acid and glucose metabolism in mice. Hence, we hypothesized that short term administration of oral antibiotics in humans would affect fecal microbiota composition and subsequently bile acid and glucose metabolism. METHODS: In this single blinded randomized controlled trial, 20 male obese subjects with metabolic syndrome were randomized to 7 days of amoxicillin 500mg t.i.d. or 7 days of vancomycin 500mg t.i.d. At baseline and after 1 week of therapy, fecal microbiota composition (Human Intestinal Tract Chip phylogenetic microarray), fecal and plasma bile acid concentrations as well as insulin sensitivity (hyperinsulinemic euglycemic clamp using [6,6-2H2]-glucose tracer) were measured. RESULTS: Vancomycin reduced fecal microbial diversity with a decrease of gram-positive bacteria (mainly Firmicutes) and a compensatory increase in gram-negative bacteria (mainly Proteobacteria). Concomitantly, vancomycin decreased fecal secondary bile acids with a simultaneous postprandial increase in primary bile acids in plasma (p

KW - salt hydrolase activity

KW - diet-induced obesity

KW - intestinal microbiota

KW - energy-expenditure

KW - mice

KW - resistance

KW - adiposity

KW - glucagon

KW - capacity

KW - humans

U2 - 10.1016/j.jhep.2013.11.034

DO - 10.1016/j.jhep.2013.11.034

M3 - Article

SN - 0168-8278

VL - 60

SP - 824

EP - 831

JO - Journal of Hepatology

JF - Journal of Hepatology

IS - 4

ER -

Impact of oral vancomycin on gut microbiota, bile acid metabolism, and insulin sensitivity

Abstract

Keywords

UN SDGs

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