Impact of anatomical traits of maize (Zea mays L.) leaf as affected by nitrogen supply and leaf age on bundle sheath conductance

The mechanism of photosynthesis in C₄ crops depends on the archetypal Kranz-anatomy. To examine how the leaf anatomy, as altered by nitrogen supply and leaf age, affects the bundle sheath conductance (g_bs), maize (Zea mays L.) plants were grown under three contrasting nitrogen levels. Combined gas exchange and chlorophyll fluorescence measurements were done on fully grown leaves at two leaf ages. The measured data were analysed using a biochemical model of C₄ photosynthesis to estimate g_bs. The leaf microstructure and ultrastructure were quantified using images obtained from micro-computed tomography and microscopy. There was a strong positive correlation between g_bs and leaf nitrogen content (LNC) while old leaves had lower g_bs than young leaves. Leaf thickness, bundle sheath cell wall thickness and surface area of bundle sheath cells per unit leaf area (S_b) correlated well with g_bs although they were not significantly affected by LNC. As a result, the increase of g_bs with LNC was little explained by the alteration of leaf anatomy. In contrast, the combined effect of LNC and leaf age on S_b was responsible for differences in g_bs between young leaves and old leaves. Future investigations should consider changes at the level of plasmodesmata and membranes along the CO₂ leakage pathway to unravel LNC and age effects further.