Immunosensor based on antibody-functionalized MoS2 for rapid detection of avian coronavirus on cotton thread

Xuan Weng, Suresh Neethirajan*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Infectious bronchitis virus (IBV), an avian coronavirus, significantly affects the performance of both the egg-laying and meat-type birds causing the foremost of economic loss in poultry industry. This paper aims to develop a rapid, low-cost, and sensitive biosensor for IBV detection by using molybdenum disulfide (MoS2). MoS2 is a 2-D nanosheet which has strong high fluorescence-quenching ability when applied to a dye-labeled antibody (Ab). In this paper, we developed an Ab-functionalized MoS2-based fluorescent immunosensor, which utilized the fluorescence resonance energy transfer (FRET) between the MoS2 and fluorescence dye during the Ab-antigen interaction. The assay was performed on a low-cost cotton thread-based microfluidic platform due to the good wicking property and flexibility. Upon the optimization of assay conditions, the immunosensor demonstrated remarkable sensitivity of 4.6× 10-2 EID50 per mL and specificity with a dynamic linear response range of 102-106 EID50 per mL for IBV standard solutions. The developed immunoassay successfully detected the IBV spiked chicken serum with satisfactory results. The foregoing presents its potential application for on-farm detection.

Original languageEnglish
Pages (from-to)4358-4363
Number of pages6
JournalIEEE Sensors Journal
Volume18
Issue number11
DOIs
Publication statusPublished - 1 Jun 2018
Externally publishedYes

Keywords

  • Cotton thread
  • FRET
  • Immunosensor
  • Infectious bronchitis virus
  • Molybdenum disulfide

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