Background: Infectious diseases are problematic for the pig sector worldwide, with the weaning period being the most critical period in a pig’s life. A promising strategy to reduce the incidence of infectious diseases is immunomodulation by feed in early life. Therefore, the immunomodulatory effects of two feed additives, yeast-derived β-glucans and E.coli Nissle 1917 (EcN), were studied in vitro and in vivo. Materials and methods: In vitro cultured dendritic cells (DCs) were used to assess the immunomodulatory effects of both feed additives. Read-out parameters included upregulation of maturation markers and production of cytokines after co-cultivation with the feed additives. For the in vivo assessment, both feed additives were administered to neonatal piglets and immune parameters (e.g. DC maturation and ex-vivo re-stimulation responses) were assessed in blood- and tissue sam-ples collected at pre- and post-weaning time points. Results: Yeast-derived β-glucans and EcN induced differential DC responses in vitro, with respect to upregulation of DC maturation markers (e.g. CD80/86) and production of cytokines (e.g. TNFα and IL-10). In line with these in vitro results, EcN promoted DC maturation during the pre-weaning phase of the in vivo study. Furthermore, ex-vivo re-stimulated immune cells from EcN treated animals produced higher amounts of the anti-inflammatory cytokine IL-10. Conclusion: These results indicate that yeast derived β-glucans and EcN demonstrated to possess immunomodulatory properties, but the type and magnitude differed. Especially EcN has the potential to enhance the porcine immune system during the pre-weaning phase, but any long lasting effects of the remain to be elucidated.
|Title of host publication||WIAS Annual Conference 2020|
|Subtitle of host publication||Frontiers in Animal Sciences|
|Publication status||Published - 14 Feb 2020|
Geervliet, M., de Vries, H. J. A., Groothuis, N., Jansen, C. A., Rutten, V., Savelkoul, H. F. J., & Tijhaar, E. J. (2020). Immunomodulatory effects of E.coli Nissle 1917 and β-glucans in early life. In WIAS Annual Conference 2020: Frontiers in Animal Sciences (pp. 36-36). WIAS.