Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

P.V. Jeurink; C. Lull Noguera; H.F.J. Savelkoul; H.J. Wichers

doi:10.1016/j.intimp.2008.04.004

Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

P.V. Jeurink, C. Lull Noguera, H.F.J. Savelkoul, H.J. Wichers

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains (Agaricus blazei, Coprinus comatus, Flammulina velutipes, Ganoderma lucidum, Grifola frondosa, Volvariella volvacea, Lentinus edodes, and Pleurotus ostreatus) were tested for the immunomodulating activity of the isolated protein fractions and polysaccharides fractions present in mycelia and culture liquid. The fungal proteins and polysaccharides have been investigated for their in vitro effect on the cytokine profile (IFN-¿, IL-4, IL-10, IL-12 and TNF-) of unstimulated or hPBMC stimulated with the polyclonal stimulations PMA/Ca-I, ConA or LPS. In addition to their influence on the cytokine profile, the hemagglutination activity of the fungal proteins on rabbit red blood cells was determined. Proteins from V. volvacea and G. lucidum showed immunomodulating activity without the presence of any mitogen, however, neither of them decreased the production of IL-4 and IFN-¿ in combination with a stimulus. All used stimuli resulted in an induction of IL-12 in the presence of the protein extracts, suggesting a direct effect on monocytes. This effect might lead to the indirect immunomodulation of T cell activation and cytokine production. In addition, both protein extracts showed more hemagglutination activity after trypsin treatment of the rabbit red blood cells, indicating the presence of carbohydrate-binding proteins, like lectins and FIPs. In conclusion, the protein extracts of V. volvacea and G. lucidum contain immunomodulating activity by acting directly on monocytes and thereby modulating T cell activation. Further purification of the fungal extracts is needed to clarify whether there are FIPs or lectins present that are responsible for this immunomodulating activity.

Original language	English
Pages (from-to)	1124-1133
Journal	International Immunopharmacology
Volume	8
Issue number	8
DOIs	https://doi.org/10.1016/j.intimp.2008.04.004
Publication status	Published - 2008

Keywords

nf-kappa-b
medicinal mushrooms
ganoderma-lucidum
in-vitro
volvariella-volvacea
edible mushroom
cancer-therapy
ling zhi-8
lectin
mycelia

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.intimp.2008.04.004

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title = "Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells",

abstract = "Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains (Agaricus blazei, Coprinus comatus, Flammulina velutipes, Ganoderma lucidum, Grifola frondosa, Volvariella volvacea, Lentinus edodes, and Pleurotus ostreatus) were tested for the immunomodulating activity of the isolated protein fractions and polysaccharides fractions present in mycelia and culture liquid. The fungal proteins and polysaccharides have been investigated for their in vitro effect on the cytokine profile (IFN-¿, IL-4, IL-10, IL-12 and TNF-) of unstimulated or hPBMC stimulated with the polyclonal stimulations PMA/Ca-I, ConA or LPS. In addition to their influence on the cytokine profile, the hemagglutination activity of the fungal proteins on rabbit red blood cells was determined. Proteins from V. volvacea and G. lucidum showed immunomodulating activity without the presence of any mitogen, however, neither of them decreased the production of IL-4 and IFN-¿ in combination with a stimulus. All used stimuli resulted in an induction of IL-12 in the presence of the protein extracts, suggesting a direct effect on monocytes. This effect might lead to the indirect immunomodulation of T cell activation and cytokine production. In addition, both protein extracts showed more hemagglutination activity after trypsin treatment of the rabbit red blood cells, indicating the presence of carbohydrate-binding proteins, like lectins and FIPs. In conclusion, the protein extracts of V. volvacea and G. lucidum contain immunomodulating activity by acting directly on monocytes and thereby modulating T cell activation. Further purification of the fungal extracts is needed to clarify whether there are FIPs or lectins present that are responsible for this immunomodulating activity.",

keywords = "nf-kappa-b, medicinal mushrooms, ganoderma-lucidum, in-vitro, volvariella-volvacea, edible mushroom, cancer-therapy, ling zhi-8, lectin, mycelia",

author = "P.V. Jeurink and {Lull Noguera}, C. and H.F.J. Savelkoul and H.J. Wichers",

year = "2008",

doi = "10.1016/j.intimp.2008.04.004",

language = "English",

volume = "8",

pages = "1124--1133",

journal = "International Immunopharmacology",

issn = "1567-5769",

publisher = "Elsevier",

number = "8",

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TY - JOUR

T1 - Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

AU - Jeurink, P.V.

AU - Lull Noguera, C.

AU - Savelkoul, H.F.J.

AU - Wichers, H.J.

PY - 2008

Y1 - 2008

N2 - Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains (Agaricus blazei, Coprinus comatus, Flammulina velutipes, Ganoderma lucidum, Grifola frondosa, Volvariella volvacea, Lentinus edodes, and Pleurotus ostreatus) were tested for the immunomodulating activity of the isolated protein fractions and polysaccharides fractions present in mycelia and culture liquid. The fungal proteins and polysaccharides have been investigated for their in vitro effect on the cytokine profile (IFN-¿, IL-4, IL-10, IL-12 and TNF-) of unstimulated or hPBMC stimulated with the polyclonal stimulations PMA/Ca-I, ConA or LPS. In addition to their influence on the cytokine profile, the hemagglutination activity of the fungal proteins on rabbit red blood cells was determined. Proteins from V. volvacea and G. lucidum showed immunomodulating activity without the presence of any mitogen, however, neither of them decreased the production of IL-4 and IFN-¿ in combination with a stimulus. All used stimuli resulted in an induction of IL-12 in the presence of the protein extracts, suggesting a direct effect on monocytes. This effect might lead to the indirect immunomodulation of T cell activation and cytokine production. In addition, both protein extracts showed more hemagglutination activity after trypsin treatment of the rabbit red blood cells, indicating the presence of carbohydrate-binding proteins, like lectins and FIPs. In conclusion, the protein extracts of V. volvacea and G. lucidum contain immunomodulating activity by acting directly on monocytes and thereby modulating T cell activation. Further purification of the fungal extracts is needed to clarify whether there are FIPs or lectins present that are responsible for this immunomodulating activity.

AB - Immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hPBMC). These activities include mitogenicity, stimulation and activation of immune effector cells. Eight mushroom strains (Agaricus blazei, Coprinus comatus, Flammulina velutipes, Ganoderma lucidum, Grifola frondosa, Volvariella volvacea, Lentinus edodes, and Pleurotus ostreatus) were tested for the immunomodulating activity of the isolated protein fractions and polysaccharides fractions present in mycelia and culture liquid. The fungal proteins and polysaccharides have been investigated for their in vitro effect on the cytokine profile (IFN-¿, IL-4, IL-10, IL-12 and TNF-) of unstimulated or hPBMC stimulated with the polyclonal stimulations PMA/Ca-I, ConA or LPS. In addition to their influence on the cytokine profile, the hemagglutination activity of the fungal proteins on rabbit red blood cells was determined. Proteins from V. volvacea and G. lucidum showed immunomodulating activity without the presence of any mitogen, however, neither of them decreased the production of IL-4 and IFN-¿ in combination with a stimulus. All used stimuli resulted in an induction of IL-12 in the presence of the protein extracts, suggesting a direct effect on monocytes. This effect might lead to the indirect immunomodulation of T cell activation and cytokine production. In addition, both protein extracts showed more hemagglutination activity after trypsin treatment of the rabbit red blood cells, indicating the presence of carbohydrate-binding proteins, like lectins and FIPs. In conclusion, the protein extracts of V. volvacea and G. lucidum contain immunomodulating activity by acting directly on monocytes and thereby modulating T cell activation. Further purification of the fungal extracts is needed to clarify whether there are FIPs or lectins present that are responsible for this immunomodulating activity.

KW - nf-kappa-b

KW - medicinal mushrooms

KW - ganoderma-lucidum

KW - in-vitro

KW - volvariella-volvacea

KW - edible mushroom

KW - cancer-therapy

KW - ling zhi-8

KW - lectin

KW - mycelia

U2 - 10.1016/j.intimp.2008.04.004

DO - 10.1016/j.intimp.2008.04.004

M3 - Article

SN - 1567-5769

VL - 8

SP - 1124

EP - 1133

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 8

ER -

Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells

Abstract

Keywords

UN SDGs

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