Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organisms and the concurrent immune response of the host

S.H.M. Jeurissen, E. Claassen, A.G. Boonstra-Blom, L. Vervelde, E.M. Janse

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    13 Citations (Scopus)

    Abstract

    For poultry as well as for mammalian species used for scientific research, many immunocytochemical techniques have been developed to investigate in detail the interaction between infectious micro-organisms and the nonspecific and specific immune systems of the host. In this review three techniques have been described with all technical details necessary to perform them correctly: (1) single immunocytochemical staining to detect the infectious micro-organisms in situ at their site of infection, (2) double immunocytochemical staining to visualize simultaneously the infectious micro- organism and the host cellular response to investigate their interactions, and (3) detection of plasma cells producing antibodies specific to the micro- organism. Of the three techniques the results are described when applied on chicken tissues infected with various micro-organisms, such as Marek's disease virus, chicken anemia virus, infectious bursal disease virus and Eimeria tenella. (C) 2000 Elsevier Science Ltd.
    Original languageEnglish
    Pages (from-to)141-151
    JournalDevelopmental and Comparative Immunology
    Volume24
    Issue number2-3
    DOIs
    Publication statusPublished - 2000

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