Immunochemical detection methods for gluten in food products: where do we go from here?

I.D. Bruins Slot, H.J. van der Fels-Klerx, Maria G.E.G. Bremer, Hamer R.J.

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Accurate and reliable quantification methods for gluten in food are necessary to ensure proper product labelling and thus safeguard the gluten sensitive consumer against exposure. Immunochemical detection is the method of choice, as it is sensitive, rapid and relatively easy to use. Although a wide range of detection kits are commercially available, there are still many difficulties in gluten detection that have not yet been overcome. This review gives an overview of the currently commercially available immunochemical detection methods, and discusses the problems that still exist in gluten detection in food. The largest problems are encountered in the extraction of gluten from food matrices, the choice of epitopes targeted by the detection method, and the use of a standardized reference material. By comparing the available techniques with the unmet needs in gluten detection, the possible benefit of a new multiplex immunoassay is investigated. This detection method would allow for the detection and quantification of multiple harmful gluten peptides at once and would, therefore, be a logical advancement in gluten detection in food.
Original languageEnglish
Pages (from-to)2455-2466
JournalCritical Reviews in Food Science and Nutrition
Volume56
Issue number15
DOIs
Publication statusPublished - 2016

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