Immune escape mutants of highly pathogenic avian influenza H5N1 selected using polyclonal sera: Identification of key amino acids in the HA protein

I. Sitaras, D. Kalthof, M. Beer, B.P.H. Peeters, M.C.M. de Jong

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Evolution of Avian Influenza (AI) viruses – especially of the Highly Pathogenic Avian Influenza (HPAI) H5N1 subtype – is a major issue for the poultry industry. HPAI H5N1 epidemics are associated with huge economic losses and are sometimes connected to human morbidity and mortality. Vaccination (either as a preventive measure or as a means to control outbreaks) is an approach that splits the scientific community, due to the risk of it being a potential driving force in HPAI evolution through the selection of mutants able to escape vaccination-induced immunity. It is therefore essential to study how mutations are selected due to immune pressure. To this effect, we performed an in vitro selection of mutants from HPAI A/turkey/Turkey/1/05 (H5N1), using immune pressure from homologous polyclonal sera. After 42 rounds of selection, we identified 5 amino acid substitutions in the Haemagglutinin (HA) protein, most of which were located in areas of antigenic importance and suspected to be prone to selection pressure. We report that most of the mutations took place early in the selection process. Finally, our antigenic cartography studies showed that the antigenic distance between the selected isolates and their parent strain increased with passage number.
Original languageEnglish
Article numbere84628
Number of pages13
JournalPLoS ONE
Volume9
Issue number2
DOIs
Publication statusPublished - 2014

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taxonomic keys
Influenza in Birds
Hemagglutinins
avian influenza
hemagglutinins
Amino Acids
mutants
amino acids
Serum
Poultry
Proteins
Pressure
proteins
vaccination
mutation
Viruses
Vaccination
early selection
poultry industry
amino acid substitution

Keywords

  • virus hemagglutinin
  • antigenic drift
  • a viruses
  • monoclonal-antibodies
  • evolution
  • poultry
  • egypt
  • vaccination
  • molecule
  • neutralization

Cite this

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title = "Immune escape mutants of highly pathogenic avian influenza H5N1 selected using polyclonal sera: Identification of key amino acids in the HA protein",
abstract = "Evolution of Avian Influenza (AI) viruses – especially of the Highly Pathogenic Avian Influenza (HPAI) H5N1 subtype – is a major issue for the poultry industry. HPAI H5N1 epidemics are associated with huge economic losses and are sometimes connected to human morbidity and mortality. Vaccination (either as a preventive measure or as a means to control outbreaks) is an approach that splits the scientific community, due to the risk of it being a potential driving force in HPAI evolution through the selection of mutants able to escape vaccination-induced immunity. It is therefore essential to study how mutations are selected due to immune pressure. To this effect, we performed an in vitro selection of mutants from HPAI A/turkey/Turkey/1/05 (H5N1), using immune pressure from homologous polyclonal sera. After 42 rounds of selection, we identified 5 amino acid substitutions in the Haemagglutinin (HA) protein, most of which were located in areas of antigenic importance and suspected to be prone to selection pressure. We report that most of the mutations took place early in the selection process. Finally, our antigenic cartography studies showed that the antigenic distance between the selected isolates and their parent strain increased with passage number.",
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author = "I. Sitaras and D. Kalthof and M. Beer and B.P.H. Peeters and {de Jong}, M.C.M.",
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Immune escape mutants of highly pathogenic avian influenza H5N1 selected using polyclonal sera: Identification of key amino acids in the HA protein. / Sitaras, I.; Kalthof, D.; Beer, M.; Peeters, B.P.H.; de Jong, M.C.M.

In: PLoS ONE, Vol. 9, No. 2, e84628, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Immune escape mutants of highly pathogenic avian influenza H5N1 selected using polyclonal sera: Identification of key amino acids in the HA protein

AU - Sitaras, I.

AU - Kalthof, D.

AU - Beer, M.

AU - Peeters, B.P.H.

AU - de Jong, M.C.M.

PY - 2014

Y1 - 2014

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KW - virus hemagglutinin

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KW - molecule

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