Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins

D. Dorokhin, W. Haasnoot, M.C.R. Franssen, H. Zuilhof, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin–protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 µg/kg for DON and 64 and 40 µg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples
Original languageEnglish
Pages (from-to)3005-3011
JournalAnalytical and Bioanalytical Chemistry
Volume400
Issue number9
DOIs
Publication statusPublished - 2011

Keywords

  • linked-immunosorbent-assay
  • fluorescence polarization immunoassay
  • tandem mass-spectrometry
  • monoclonal-antibodies
  • liquid-chromatography
  • gas-chromatography
  • rapid detection
  • deoxynivalenol
  • wheat
  • toxin

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