Abstract
A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin–protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 µg/kg for DON and 64 and 40 µg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples
Original language | English |
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Pages (from-to) | 3005-3011 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 400 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2011 |
Keywords
- linked-immunosorbent-assay
- fluorescence polarization immunoassay
- tandem mass-spectrometry
- monoclonal-antibodies
- liquid-chromatography
- gas-chromatography
- rapid detection
- deoxynivalenol
- wheat
- toxin