Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Inflection in Rats

M.T.J. van Ampting; G.C.H. Rodenburg; C. Vink; E. Kramer; A. Schonewille; J. Keijer; R. van der Meer; I.M.J. Bovee-Oudenhoven

doi:10.1007/s10620-008-0685-0

Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Inflection in Rats

M.T.J. van Ampting, G.C.H. Rodenburg, C. Vink, E. Kramer, A. Schonewille, J. Keijer, R. van der Meer, I.M.J. Bovee-Oudenhoven

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

Background Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection. Results Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces. Conclusions PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker

Original language	English
Pages (from-to)	2588-2597
Journal	Digestive Diseases and Sciences
Volume	54
Issue number	12
DOIs	https://doi.org/10.1007/s10620-008-0685-0
Publication status	Published - 2009

Keywords

inflammatory-bowel-disease
dietary fructo-oligosaccharides
enterotoxigenic escherichia-coli
gene-expression
intestinal colonization
bacterial-colonization
stone protein
nitric-oxide
reg gene
calcium

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10.1007/s10620-008-0685-0

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@article{ee27e708dd874b2ca4bd753bdb8a3023,

title = "Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Inflection in Rats",

abstract = "Background Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection. Results Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces. Conclusions PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker",

keywords = "inflammatory-bowel-disease, dietary fructo-oligosaccharides, enterotoxigenic escherichia-coli, gene-expression, intestinal colonization, bacterial-colonization, stone protein, nitric-oxide, reg gene, calcium",

author = "{van Ampting}, M.T.J. and G.C.H. Rodenburg and C. Vink and E. Kramer and A. Schonewille and J. Keijer and {van der Meer}, R. and I.M.J. Bovee-Oudenhoven",

year = "2009",

doi = "10.1007/s10620-008-0685-0",

language = "English",

volume = "54",

pages = "2588--2597",

journal = "Digestive Diseases and Sciences",

issn = "0163-2116",

publisher = "Springer",

number = "12",

}

TY - JOUR

T1 - Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Inflection in Rats

AU - van Ampting, M.T.J.

AU - Rodenburg, G.C.H.

AU - Vink, C.

AU - Kramer, E.

AU - Schonewille, A.

AU - Keijer, J.

AU - van der Meer, R.

AU - Bovee-Oudenhoven, I.M.J.

PY - 2009

Y1 - 2009

N2 - Background Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection. Results Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces. Conclusions PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker

AB - Background Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection. Results Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces. Conclusions PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker

KW - inflammatory-bowel-disease

KW - dietary fructo-oligosaccharides

KW - enterotoxigenic escherichia-coli

KW - gene-expression

KW - intestinal colonization

KW - bacterial-colonization

KW - stone protein

KW - nitric-oxide

KW - reg gene

KW - calcium

U2 - 10.1007/s10620-008-0685-0

DO - 10.1007/s10620-008-0685-0

M3 - Article

SN - 0163-2116

VL - 54

SP - 2588

EP - 2597

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 12

ER -

Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Inflection in Rats

Abstract

Keywords

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