Ileal endogenous nitrogen recovery is increased and its amino acid pattern is altered in pigs fed quebracho extract

C.A. Steendam, S. Tamminga, H. Boer, E.J. de Jong, G.H. Visser, M.W.A. Verstegen

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Abstract

Ileal endogenous nitrogen recovery (ENR) in pigs (9 ± 0.6 kg body weight) was estimated simultaneously using the 15N-isotope dilution technique (15N-IDT) and the peptide alimentation ultrafiltration (UF) method. Diets were cornstarch, enzyme-hydrolyzed casein with no (control) or high (4%) content of quebracho extract (Schinopsis spp.) rich in condensed tannins. The amino acid (AA) pattern of the ENR was also determined. The ENR of pigs fed the quebracho diet was higher (P = 0.0001) than that of pigs fed the control diet [6.00 vs. 1.95 g/kg dry matter intake (DMI) for the 15N-IDT and 5.18 vs. 1.49 g/kg DMI for the UF method, respectively]. With the 15N-IDT, ENR values were 0.44-0.79 g/kg DMI (24%) higher (control P = 0.0032, quebracho P = 0.0002) than for the UF method. Apparent nitrogen digestibility depended on diet (69.0% quebracho vs. 86.0% control, P = 0.0001). Real nitrogen digestibility (RD-N) determined by the UF method was higher (P = 0.0001) for the control than for the quebracho diet (91.4 vs. 88.2%). Corresponding values for the 15N-IDT did not differ (P = 0.0569) between diets (92.8 vs. 91.4%). The 15N-IDT gave higher values for RD-N of both diets (control P = 0.0030, quebracho P = 0.0002) compared with the UF method. Endogenous AA recoveries (g/kg DMI) were increased 300% (P = 0.0001) and the AA-pattern of ENR was changed (P from 0.0001 to 0.7530 for different AA) by the quebracho diet. A constant AA-pattern of ENR cannot be assumed. Despite limitations of both techniques, the 15N-IDT and the UF method gave similar results with respect to ENR
Original languageEnglish
Pages (from-to)3076-3082
JournalThe Journal of Nutrition
Volume134
Issue number11
DOIs
Publication statusPublished - Nov 2004

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Keywords

  • n-15-isotope dilution technique
  • hydrolyzed protein method
  • kg liveweight pig
  • growing pig
  • peptide alimentation
  • condensed tannins
  • terminal ileum
  • free diet
  • rat
  • excretion

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