IgE Cross-Reactivity of Cashew Nut Allergens

Shanna Bastiaan-Net*, Marit Reitsma, Jan H.G. Cordewener, Johanna P.M. van der Valk, Twan A.H.P. America, Anthony E.J. Dubois, Roy Gerth van Wijk, Huub F.J. Savelkoul, Nicolette W. de Jong, Harry J. Wichers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Background: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. Objectives: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. Method: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. Results: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63%). Of these, 50% were mono-sensitised to cashew nuts, 19% were co-sensitised to Anacardiaceae species, and 31% were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. Conclusions: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.

Original languageEnglish
Pages (from-to)19-32
JournalInternational Archives of Allergy and Immunology
Volume178
Issue number1
Early online date26 Oct 2018
DOIs
Publication statusPublished - 2019

Fingerprint

Anacardium
Nuts
Allergens
Immunoglobulin E
Anacardiaceae
Rhus
Pistacia
Mangifera
Nut Hypersensitivity
Reactive Inhibition
Seed Storage Proteins
Aptitude

Keywords

  • Allergenicity
  • Cashew nut
  • Food allergy
  • IgE cross-reactivity
  • Immunoblotting
  • Tree nut allergy

Cite this

Bastiaan-Net, Shanna ; Reitsma, Marit ; Cordewener, Jan H.G. ; van der Valk, Johanna P.M. ; America, Twan A.H.P. ; Dubois, Anthony E.J. ; Gerth van Wijk, Roy ; Savelkoul, Huub F.J. ; de Jong, Nicolette W. ; Wichers, Harry J. / IgE Cross-Reactivity of Cashew Nut Allergens. In: International Archives of Allergy and Immunology. 2019 ; Vol. 178, No. 1. pp. 19-32.
@article{8a38af844c7d4b98a487008789192096,
title = "IgE Cross-Reactivity of Cashew Nut Allergens",
abstract = "Background: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. Objectives: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. Method: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. Results: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63{\%}). Of these, 50{\%} were mono-sensitised to cashew nuts, 19{\%} were co-sensitised to Anacardiaceae species, and 31{\%} were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. Conclusions: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.",
keywords = "Allergenicity, Cashew nut, Food allergy, IgE cross-reactivity, Immunoblotting, Tree nut allergy",
author = "Shanna Bastiaan-Net and Marit Reitsma and Cordewener, {Jan H.G.} and {van der Valk}, {Johanna P.M.} and America, {Twan A.H.P.} and Dubois, {Anthony E.J.} and {Gerth van Wijk}, Roy and Savelkoul, {Huub F.J.} and {de Jong}, {Nicolette W.} and Wichers, {Harry J.}",
year = "2019",
doi = "10.1159/000493100",
language = "English",
volume = "178",
pages = "19--32",
journal = "International Archives of Allergy and Immunology",
issn = "1018-2438",
publisher = "Karger",
number = "1",

}

IgE Cross-Reactivity of Cashew Nut Allergens. / Bastiaan-Net, Shanna; Reitsma, Marit; Cordewener, Jan H.G.; van der Valk, Johanna P.M.; America, Twan A.H.P.; Dubois, Anthony E.J.; Gerth van Wijk, Roy; Savelkoul, Huub F.J.; de Jong, Nicolette W.; Wichers, Harry J.

In: International Archives of Allergy and Immunology, Vol. 178, No. 1, 2019, p. 19-32.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - IgE Cross-Reactivity of Cashew Nut Allergens

AU - Bastiaan-Net, Shanna

AU - Reitsma, Marit

AU - Cordewener, Jan H.G.

AU - van der Valk, Johanna P.M.

AU - America, Twan A.H.P.

AU - Dubois, Anthony E.J.

AU - Gerth van Wijk, Roy

AU - Savelkoul, Huub F.J.

AU - de Jong, Nicolette W.

AU - Wichers, Harry J.

PY - 2019

Y1 - 2019

N2 - Background: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. Objectives: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. Method: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. Results: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63%). Of these, 50% were mono-sensitised to cashew nuts, 19% were co-sensitised to Anacardiaceae species, and 31% were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. Conclusions: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.

AB - Background: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. Objectives: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. Method: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. Results: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63%). Of these, 50% were mono-sensitised to cashew nuts, 19% were co-sensitised to Anacardiaceae species, and 31% were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. Conclusions: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.

KW - Allergenicity

KW - Cashew nut

KW - Food allergy

KW - IgE cross-reactivity

KW - Immunoblotting

KW - Tree nut allergy

U2 - 10.1159/000493100

DO - 10.1159/000493100

M3 - Article

VL - 178

SP - 19

EP - 32

JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

SN - 1018-2438

IS - 1

ER -