Identification of the major constituents of Hypericum perforatum by LC/SPE/NMR and/or LC/MS.

E.C. Tatsis, J.A. Boeren, V. Exarchou, A.N. Troganis, J.J.M. Vervoort, I.P. Gerothanassis

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222 Citations (Scopus)


The newly established hyphenated instrumentation of LC/DAD/SPE/NMR and LC/UV/(ESI)MS techniques have been applied for separation and structure verification of the major known constituents present in Greek Hypericum perforatum extracts. The chromatographic separation was performed on a C18 column. Acetonitrile-water was used as a mobile phase. For the on-line NMR detection, the analytes eluted from column were trapped one by one onto separate SPE cartridges, and hereafter transported into the NMR flow-cell. LC/DAD/SPE/NMR and LC/UV/MS allowed the characterization of constituents of Greek H. perforatum, mainly naphtodianthrones (hypericin, pseudohypericin, protohypericin, protopseudohypericin), phloroglucinols (hyperforin, adhyperforin), flavonoids (quercetin, quercitrin, isoquercitrin, hyperoside, astilbin, miquelianin, I3,II8-biapigenin) and phenolic acids (chlorogenic acid, 3-O-coumaroylquinic acid). Two phloroglucinols (hyperfirin and adhyperfirin) were detected for the first time, which have been previously reported to be precursors in the biosynthesis of hyperforin and adhyperforin
Original languageEnglish
Pages (from-to)383-393
Issue number3
Publication statusPublished - 2007


  • st-johns-wort
  • performance liquid-chromatography
  • nuclear-magnetic-resonance
  • solid-phase extraction
  • dad-spe-nmr
  • mass-spectrometry
  • phenolic composition
  • array detection
  • plant-extracts
  • greek oregano


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