Identification of the connecting linkage between homo- or xylogalacturonan and rhamnogalacturonan type I

G.J. Coenen, E.J. Bakx, R.P. Verhoef, H.A. Schols, A.G.J. Voragen

Research output: Contribution to journalArticleAcademicpeer-review

113 Citations (Scopus)

Abstract

Pectin is of interest both as cell wall component and as food additive. The precise chemical structure of pectin remains under debate, although the structural elements of pectin are rather well described. In order to get more insight in the inter linkage between the various structural elements, apple pectin modified hairy regions were degraded by controlled acid hydrolysis. From the degradation products oligomeric fragments were selected which could represent interconnection points, and these oligomers were characterized using LC¿MS and NMR approaches. It was shown that the oligomers GalA3Rha1, GalA4Rha2, and GalA5Rha3 consisted out of a homogalacturonan and a rhamnogalacturonan type I segment connected via a GalAp¿-(1 ¿ 2) Rhap linkage. In addition, a GalA6Rha3Xyl1 oligomer was identified, which consisted out of a xylogalacturonan and a rhamnogalacturonan type I segment. These oligomers indicated that in apple pectin both homogalacturonan and xylogalacturonan were covalently linked to rhamnogalacturonan type I. With these new insights, currently used pectin models were refined. Keywords: Homogalacturonan; Xylogalacturonan; Rhamnogalacturonan I; Pectin model; Covalent linkage; Pectin structure
Original languageEnglish
Pages (from-to)224-235
JournalCarbohydrate Polymers
Volume70
Issue number2
DOIs
Publication statusPublished - 2007

Keywords

  • hairy ramified regions
  • cell-wall polysaccharides
  • flight mass-spectrometry
  • pectic polysaccharide
  • structural features
  • rhamnose residues
  • black-currants
  • cross-linking
  • apple pectin
  • side-chain

Fingerprint Dive into the research topics of 'Identification of the connecting linkage between homo- or xylogalacturonan and rhamnogalacturonan type I'. Together they form a unique fingerprint.

Cite this