Identification of sugarcane genes induced in disease-resistant somaclones upon inoculation with Ustilago scitaminea or Bipolaris sacchari

O. Borrás-Hidalgo, B.P.H.J. Thomma, E. Carmona, C.J. Borroto, M. Pujol, A. Arencibia, J. Lopez

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)

Abstract

To understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant genes that respond to the pathogen attack. Amplified fragment length polymorphism (AFLP) analysis of cDNA was used to identify sugarcane genes differentially expressed in disease-resistant but not in susceptible sugarcane somaclones in response to inoculation with either Ustilago scitaminea or Bipolaris sacchari (also known as Helminthosporium sacchari or Drechslera sacchari), causal agents of smut and eyespot respectively. In total 62 differentially regulated genes were identified, of which 10 were down-regulated and 52 were induced. Of these 52, 19 transcript derived fragments showed homology to known plant gene sequences, most of them related to defense or signaling. The total set of differentially expressed sugarcane genes can be an important resource for further studies aimed at understanding sugarcane pathogen defense
Original languageEnglish
Pages (from-to)1115-1121
JournalPlant Physiology and Biochemistry
Volume43
Issue number12
DOIs
Publication statusPublished - 2005

Keywords

  • phenylalanine ammonia-lyase
  • lipid-transfer proteins
  • tobacco-mosaic-virus
  • salicylic-acid
  • arabidopsis
  • expression
  • infection
  • patatin
  • plants
  • cdna

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