Identification of Radical Scavenging Compounds in Rhaponticum carthamoides by Means of LC-DAD-SPE-NMR

G. Miliauskas, T.A. van Beek, P. de Waard, R.P. Venskutonis, E.J.R. Sudhölter

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73 Citations (Scopus)


A hyphenated LC-DAD-SPE-NMR setup in combination with on-line radical scavenging detection has been applied for the identification of radical scavenging compounds in extracts of Rhaponticum carthamoides. After NMR measurements, the pure compounds were infused into a mass spectrometer. The technique enabled selective detection and identification of individual radical scavenging compounds without any prior off-line chromatographic steps. Seven compounds, namely, quercetagetin-7--glucopyranoside (1), quercetagetin-7-(6' '-acetyl--glucopyranoside) (3), 6-hydroxykaempferol-7--glucopyranoside (2), 6-methoxykaempferol-3--glucopyranoside (4), 6-hydroxykaempferol-7-(6' '-acetyl--glucopyranoside) (5), chlorogenic acid (6), and -ecdysone (7), were identified in ethanol or aqueous extracts. Compound 5 is a new natural compound. Its radical scavenging activity was tested against DPPH radical and was found to be weaker than that of the reference antioxidants rosmarinic acid and Trolox
Original languageEnglish
Pages (from-to)168-172
JournalJournal of Natural Products
Issue number2
Publication statusPublished - 2005


  • antioxidant activity
  • glycosides
  • leaves

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