Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4

H.J. Finkers, A.W. van Heusden, A.J. van der Have, J.A.L. van Kan, P. Lindhout

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

BOTRYTIS cinerea Pers:Fr (teleomorph: Botryotina fuckeliana (de Bary) Whetzel) is a necrotrophic pathogenic fungus with an exceptionally wide host range of at least 235 species. Modern hybrid tomato (Solanum lycopersicum) cultivars are susceptible to B. cinerea although there are cultivars with some quantitative resistance. In a screening Solanum habrochaites LYC4 showed a good level of resistance. To study the genetics behind this resistance, an F2 population (n=174) of the cross between a susceptible genotype S. lycopersicum cv. Moneymaker and S. habrochaites LYC4 was developed. Initially, two QTLs for resistance to B. cinerea were identified (QTL1 and QTL2). During the confirmation of these two QTLs in segregating BC2S1 families, a third QTL (QTL3) was identified from which the effect could only be observed in absence of QTL2. A set of QTL specific CAPS makers was developed as a tool for marker assisted selection (MAS). Using this set of markers, one BC2S1 line was selected which was homozygous for both QTL 1 and QTL3. BC2S2 offspring of this line was tested for resistance to B. cinerea in a greenhouse trail. In the selected BC2S2 offspring only 15% of the infections were successfully established (disease incidence). This is significantly less than the susceptible control S. lycopersicum (disease incidence 70%); a commercial cultivar known as fairly resistant still had a disease incidence of 50%. This result shows that the QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.
Original languageEnglish
Title of host publicationBiology of plant-microbe interactions
EditorsFederico Sanchez, Carmen Quinto, Isabel M. Lopez-Lara, Otto Geiger
Place of PublicationSt. Paul, Minnesota
PublisherInternational Society for Molecular Plant-Microbe Interactions
Pages625-629
Number of pages717
ISBN (Print)9780965462549
Publication statusPublished - 2006
Event12th Int. Congress on Molecular Plant-Microbe Interactions -
Duration: 14 Dec 200519 Dec 2005

Conference

Conference12th Int. Congress on Molecular Plant-Microbe Interactions
Period14/12/0519/12/05

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Botrytis cinerea
quantitative trait loci
Solanum habrochaites
Solanum lycopersicum
disease incidence
cultivars
tomatoes
genetic resistance
marker-assisted selection
host range
screening
greenhouses
fungi
genotype
breeding
infection

Cite this

Finkers, H. J., van Heusden, A. W., van der Have, A. J., van Kan, J. A. L., & Lindhout, P. (2006). Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4. In F. Sanchez, C. Quinto, I. M. Lopez-Lara, & O. Geiger (Eds.), Biology of plant-microbe interactions (pp. 625-629). St. Paul, Minnesota: International Society for Molecular Plant-Microbe Interactions.
Finkers, H.J. ; van Heusden, A.W. ; van der Have, A.J. ; van Kan, J.A.L. ; Lindhout, P. / Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4. Biology of plant-microbe interactions. editor / Federico Sanchez ; Carmen Quinto ; Isabel M. Lopez-Lara ; Otto Geiger. St. Paul, Minnesota : International Society for Molecular Plant-Microbe Interactions, 2006. pp. 625-629
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title = "Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4",
abstract = "BOTRYTIS cinerea Pers:Fr (teleomorph: Botryotina fuckeliana (de Bary) Whetzel) is a necrotrophic pathogenic fungus with an exceptionally wide host range of at least 235 species. Modern hybrid tomato (Solanum lycopersicum) cultivars are susceptible to B. cinerea although there are cultivars with some quantitative resistance. In a screening Solanum habrochaites LYC4 showed a good level of resistance. To study the genetics behind this resistance, an F2 population (n=174) of the cross between a susceptible genotype S. lycopersicum cv. Moneymaker and S. habrochaites LYC4 was developed. Initially, two QTLs for resistance to B. cinerea were identified (QTL1 and QTL2). During the confirmation of these two QTLs in segregating BC2S1 families, a third QTL (QTL3) was identified from which the effect could only be observed in absence of QTL2. A set of QTL specific CAPS makers was developed as a tool for marker assisted selection (MAS). Using this set of markers, one BC2S1 line was selected which was homozygous for both QTL 1 and QTL3. BC2S2 offspring of this line was tested for resistance to B. cinerea in a greenhouse trail. In the selected BC2S2 offspring only 15{\%} of the infections were successfully established (disease incidence). This is significantly less than the susceptible control S. lycopersicum (disease incidence 70{\%}); a commercial cultivar known as fairly resistant still had a disease incidence of 50{\%}. This result shows that the QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.",
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year = "2006",
language = "English",
isbn = "9780965462549",
pages = "625--629",
editor = "Federico Sanchez and Carmen Quinto and Lopez-Lara, {Isabel M.} and Otto Geiger",
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Finkers, HJ, van Heusden, AW, van der Have, AJ, van Kan, JAL & Lindhout, P 2006, Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4. in F Sanchez, C Quinto, IM Lopez-Lara & O Geiger (eds), Biology of plant-microbe interactions. International Society for Molecular Plant-Microbe Interactions, St. Paul, Minnesota, pp. 625-629, 12th Int. Congress on Molecular Plant-Microbe Interactions, 14/12/05.

Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4. / Finkers, H.J.; van Heusden, A.W.; van der Have, A.J.; van Kan, J.A.L.; Lindhout, P.

Biology of plant-microbe interactions. ed. / Federico Sanchez; Carmen Quinto; Isabel M. Lopez-Lara; Otto Geiger. St. Paul, Minnesota : International Society for Molecular Plant-Microbe Interactions, 2006. p. 625-629.

Research output: Chapter in Book/Report/Conference proceedingConference paper

TY - GEN

T1 - Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4

AU - Finkers, H.J.

AU - van Heusden, A.W.

AU - van der Have, A.J.

AU - van Kan, J.A.L.

AU - Lindhout, P.

PY - 2006

Y1 - 2006

N2 - BOTRYTIS cinerea Pers:Fr (teleomorph: Botryotina fuckeliana (de Bary) Whetzel) is a necrotrophic pathogenic fungus with an exceptionally wide host range of at least 235 species. Modern hybrid tomato (Solanum lycopersicum) cultivars are susceptible to B. cinerea although there are cultivars with some quantitative resistance. In a screening Solanum habrochaites LYC4 showed a good level of resistance. To study the genetics behind this resistance, an F2 population (n=174) of the cross between a susceptible genotype S. lycopersicum cv. Moneymaker and S. habrochaites LYC4 was developed. Initially, two QTLs for resistance to B. cinerea were identified (QTL1 and QTL2). During the confirmation of these two QTLs in segregating BC2S1 families, a third QTL (QTL3) was identified from which the effect could only be observed in absence of QTL2. A set of QTL specific CAPS makers was developed as a tool for marker assisted selection (MAS). Using this set of markers, one BC2S1 line was selected which was homozygous for both QTL 1 and QTL3. BC2S2 offspring of this line was tested for resistance to B. cinerea in a greenhouse trail. In the selected BC2S2 offspring only 15% of the infections were successfully established (disease incidence). This is significantly less than the susceptible control S. lycopersicum (disease incidence 70%); a commercial cultivar known as fairly resistant still had a disease incidence of 50%. This result shows that the QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.

AB - BOTRYTIS cinerea Pers:Fr (teleomorph: Botryotina fuckeliana (de Bary) Whetzel) is a necrotrophic pathogenic fungus with an exceptionally wide host range of at least 235 species. Modern hybrid tomato (Solanum lycopersicum) cultivars are susceptible to B. cinerea although there are cultivars with some quantitative resistance. In a screening Solanum habrochaites LYC4 showed a good level of resistance. To study the genetics behind this resistance, an F2 population (n=174) of the cross between a susceptible genotype S. lycopersicum cv. Moneymaker and S. habrochaites LYC4 was developed. Initially, two QTLs for resistance to B. cinerea were identified (QTL1 and QTL2). During the confirmation of these two QTLs in segregating BC2S1 families, a third QTL (QTL3) was identified from which the effect could only be observed in absence of QTL2. A set of QTL specific CAPS makers was developed as a tool for marker assisted selection (MAS). Using this set of markers, one BC2S1 line was selected which was homozygous for both QTL 1 and QTL3. BC2S2 offspring of this line was tested for resistance to B. cinerea in a greenhouse trail. In the selected BC2S2 offspring only 15% of the infections were successfully established (disease incidence). This is significantly less than the susceptible control S. lycopersicum (disease incidence 70%); a commercial cultivar known as fairly resistant still had a disease incidence of 50%. This result shows that the QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.

M3 - Conference paper

SN - 9780965462549

SP - 625

EP - 629

BT - Biology of plant-microbe interactions

A2 - Sanchez, Federico

A2 - Quinto, Carmen

A2 - Lopez-Lara, Isabel M.

A2 - Geiger, Otto

PB - International Society for Molecular Plant-Microbe Interactions

CY - St. Paul, Minnesota

ER -

Finkers HJ, van Heusden AW, van der Have AJ, van Kan JAL, Lindhout P. Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4. In Sanchez F, Quinto C, Lopez-Lara IM, Geiger O, editors, Biology of plant-microbe interactions. St. Paul, Minnesota: International Society for Molecular Plant-Microbe Interactions. 2006. p. 625-629