Identification of QTLs for Botrytis cinerea Resistance in S. Habrochaites LYC4

H.J. Finkers, A.W. van Heusden, A.J. van der Have, J.A.L. van Kan, P. Lindhout

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

BOTRYTIS cinerea Pers:Fr (teleomorph: Botryotina fuckeliana (de Bary) Whetzel) is a necrotrophic pathogenic fungus with an exceptionally wide host range of at least 235 species. Modern hybrid tomato (Solanum lycopersicum) cultivars are susceptible to B. cinerea although there are cultivars with some quantitative resistance. In a screening Solanum habrochaites LYC4 showed a good level of resistance. To study the genetics behind this resistance, an F2 population (n=174) of the cross between a susceptible genotype S. lycopersicum cv. Moneymaker and S. habrochaites LYC4 was developed. Initially, two QTLs for resistance to B. cinerea were identified (QTL1 and QTL2). During the confirmation of these two QTLs in segregating BC2S1 families, a third QTL (QTL3) was identified from which the effect could only be observed in absence of QTL2. A set of QTL specific CAPS makers was developed as a tool for marker assisted selection (MAS). Using this set of markers, one BC2S1 line was selected which was homozygous for both QTL 1 and QTL3. BC2S2 offspring of this line was tested for resistance to B. cinerea in a greenhouse trail. In the selected BC2S2 offspring only 15% of the infections were successfully established (disease incidence). This is significantly less than the susceptible control S. lycopersicum (disease incidence 70%); a commercial cultivar known as fairly resistant still had a disease incidence of 50%. This result shows that the QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.
Original languageEnglish
Title of host publicationBiology of plant-microbe interactions
EditorsFederico Sanchez, Carmen Quinto, Isabel M. Lopez-Lara, Otto Geiger
Place of PublicationSt. Paul, Minnesota
PublisherInternational Society for Molecular Plant-Microbe Interactions
Pages625-629
Number of pages717
ISBN (Print)9780965462549
Publication statusPublished - 2006
Event12th Int. Congress on Molecular Plant-Microbe Interactions -
Duration: 14 Dec 200519 Dec 2005

Conference

Conference12th Int. Congress on Molecular Plant-Microbe Interactions
Period14/12/0519/12/05

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