Identification of QTLs controlling symptom development during viral infection in Arabidopsis thaliana

O. Sicard, O. Loudet, J.J.B. Keurentjes, V. Candresse, O. Le Gall, F. Revers, V. Decroocq

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana¿Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. In particular, Cvi-1 (Cape Verde islands) accumulates viral particules but remains symptomless, Col-0 (Columbia) sometimes shows weak symptoms compared with Ler (Landsberg erecta), which always shows severe symptoms. Genetic analyses of Col × Ler and Cvi × Ler F2 and recombinant inbred line (RIL) populations suggested that symptom development as well as viral accumulation traits are polygenic and quantitative. Three of the symptom quantitative trait loci (QTL) identified could be confirmed in near-isogenic lines, including PSI1 (PPV symptom induction 1), which was identified on the distal part of chromosome 1 in both RIL populations. With respect to viral accumulation, several factors have been detected and, interestingly, in the Col × Ler population, two out of three viral accumulation QTL colocalized with loci controlling symptom development, although correlation analysis showed weak linearity between symptom severity and virus accumulation. In addition, in the Cvi × Ler RIL population, a digenic recessive determinant controlling PPV infection was identified
Original languageEnglish
Pages (from-to)198-207
JournalMolecular Plant-Microbe Interactions
Volume21
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • natural allelic variation
  • inbred line population
  • lettuce-mosaic-virus
  • plum-pox-virus
  • mottle virus
  • protein gene
  • linkage maps
  • resistance
  • host
  • movement

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