Identification of potential serum biomarkers of inflammation and lipid modulation that are altered by fish oil supplementation in healthy volunteers

B. de Roos, A. Geelen, K. Ross, G. Rucklidge, M. Reid, G. Duncan, M.J. Caslake, G. Horgan, I.A. Brouwer

Research output: Contribution to journalArticleAcademicpeer-review

71 Citations (Scopus)

Abstract

Long chain n-3 polyunsaturated fatty acids (n-3 LCPUFA) lower risk of coronary heart disease (CHD), but mechanisms are not well understood. We used proteomics to identify human serum proteins that are altered by n-3 LCPUFA. Such proteins could identify pathways whereby they affect CHD. Eighty-one healthy volunteers entered a double blind randomised trial to receive 3.5 g of fish oil or 3.5 g of high oleic sunflower oil daily. Serum was collected before and after 6 wk of intervention. Serum was analysed by proteomics using 2-DE. Proteins that were differentially regulated were identified by MS. We also analysed serum apolipoprotein A1 (apo A1), high-density lipoprotein (HDL) particle size and haptoglobin. Serum levels of apo A1, apo L1, zinc--2-glycoprotein, haptoglobin precursor, -1-antitrypsin precursor, antithrombin III-like protein, serum amyloid P component and haemopexin were significantly downregulated (all p
Original languageEnglish
Pages (from-to)1965-1974
JournalProteomics
Volume8
Issue number10
DOIs
Publication statusPublished - 2008

Keywords

  • tumor-necrosis-factor
  • n-3 fatty-acids
  • high-density-lipoprotein
  • human endothelial-cells
  • acute-phase response
  • eicosapentaenoic acid
  • dietary supplementation
  • plasma triglycerides
  • mobilizing factor
  • factor-alpha

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